Compartmental specificity of cellular membrane fusion encoded in SNARE proteins Journal Article
| Authors: | Mcnew, J. A.; Parlati, F.; Fukuda, R.; Johnston, R. J.; Paz, K.; Paumet, F.; Söllner, T. H.; Rothman, J. E. |
| Article Title: | Compartmental specificity of cellular membrane fusion encoded in SNARE proteins |
| Abstract: | Membrane-enveloped vesicles travel among the compartments of the cytoplasm of eukaryotic cells, delivering their specific cargo to programmed locations by membrane fusion. The palring of vesicle v-SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) with target membrane t-SNAREs has a central role in intracellular membrane fusion. We have tested all of the potential v-SNAREs encoded in the yeast genome for their capacity to trigger fusion by partnering with t-SNAREs that mark the Golgi, the vacuole and the plasma membrane. Here we find that, to a marked degree, the pattern of membrane flow in the cell is encoded and recapitulated by its isolated SNARE proteins, as predicted by the SNARE hypothesis. |
| Keywords: | protein expression; nonhuman; protein analysis; membrane proteins; endoplasmic reticulum; saccharomyces cerevisiae; eukaryota; escherichia coli; recombinant proteins; cell membrane; saccharomyces cerevisiae proteins; biological transport; liposomes; golgi complex; cell vacuole; fungal proteins; vesicular transport proteins; intracellular membranes; membrane fusion; snare protein; qa-snare proteins; golgi apparatus; snare proteins; priority journal; article; cell compartmentation; qc-snare proteins |
| Journal Title: | Nature |
| Volume: | 407 |
| Issue: | 6801 |
| ISSN: | 0028-0836 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2000-09-14 |
| Start Page: | 153 |
| End Page: | 159 |
| Language: | English |
| DOI: | 10.1038/35025000 |
| PUBMED: | 11001046 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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