Engagement of bone morphogenetic protein type IB receptor and Smad1 signaling by anti-Mullerian hormone and its type II receptor Journal Article
| Authors: | Gouédard, L.; Chen, Y. G.; Thevenet, L.; Racine, C.; Borie, S.; Lamarre, I.; Josso, N.; Massagué, J.; Di Clemente, N. |
| Article Title: | Engagement of bone morphogenetic protein type IB receptor and Smad1 signaling by anti-Mullerian hormone and its type II receptor |
| Abstract: | Anti-Mullerian hormone induces the regression of fetal Mullerian ducts and inhibits the transcription of gonadal steroidogenic enzymes. It belongs to the transforming growth factor-β family whose members signal through a pair of serine/threonine kinase receptors and Smad effectors. Only the anti-Mullerian hormone type II receptor has been identified. Our goal was to determine whether anti-Mullerian hormone could share a type I receptor with another family member. Co-immunoprecipitation of known type I receptors with anti-Mullerian hormone type II receptor clearly showed that the bone morphogenetic protein type IB receptor was the only cloned type I receptor interacting in a ligand-dependent manner with this type II receptor. Anti-Mullerian hormone also activates the bone morphogenetic protein-specific Smad1 pathway and the XVent2 reporter gene, an anti-Mullerian hormone type II receptor-dependent effect abrogated by a dominant negative version of bone morphogenetic protein type IB receptor. Reverse amplification experiments showed that bone morphogenetic protein type IB receptor is co-expressed with anti-Mullerian hormone type II receptor in most anti-Mullerian hormone target tissues. Our data support a model in which a ligand, anti-Mullerian hormone, gains access to a shared type I receptor and Smad1 system through a highly restricted type II receptor. |
| Keywords: | signal transduction; dna-binding proteins; nonhuman; proteins; animal cell; mouse; animals; mice; bone morphogenetic protein receptor; smad protein; smad1 protein; transforming growth factor beta; protein protein interaction; cell line; tumor cells, cultured; transfection; protein serine threonine kinase; animalia; gene activation; transcription regulation; reverse transcriptase polymerase chain reaction; protein-serine-threonine kinases; recombinant proteins; immunoprecipitation; reporter gene; smad proteins; genes, reporter; trans-activators; glycoproteins; muellerian duct; receptors, cell surface; receptors, growth factor; muellerian inhibiting factor; cho cells; cricetinae; growth inhibitors; hormone receptor interaction; receptors, peptide; bone morphogenetic protein receptors, type i; humans; priority journal; article; bone morphogenetic protein receptors, type ii; testicular hormones |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 275 |
| Issue: | 36 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2000-09-08 |
| Start Page: | 27973 |
| End Page: | 27978 |
| Language: | English |
| DOI: | 10.1074/jbc.M002704200 |
| PUBMED: | 10854429 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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