Detecting gene copy number fluctuations in tumor cells by microarray analysis of genomic representations Journal Article
| Authors: | Lucito, R.; West, J.; Reiner, A.; Alexander, J.; Esposito, D.; Mishra, B.; Powers, S.; Norton, L.; Wigler, M. |
| Article Title: | Detecting gene copy number fluctuations in tumor cells by microarray analysis of genomic representations |
| Abstract: | In this work, we explore the use of representations in conjunction with DNA microarray technology to measure gene copy number changes in cancer. We demonstrate that arrays of DNA probes derived from low-complexity representations can be used to detect amplifications, deletions, and polymorphic differences when hybridized to representations of genomic DNA. The method is both reproducible and verifiable, and is applicable even to microscopic amounts of primary tumors. We also present a mathematical model for array performance that is useful for designing and understanding DNA microarray hybridization protocols. The future applications and challenges of this approach are discussed. |
| Keywords: | controlled study; human cell; gene deletion; nonhuman; cancer diagnosis; diagnostic accuracy; genetic analysis; reproducibility; reproducibility of results; breast cancer; gene amplification; gene expression profiling; tumor biopsy; cancer cell culture; tumor cells, cultured; breast neoplasms; mathematical model; dna; diagnostic value; oligonucleotide array sequence analysis; escherichia coli; tumor cell; models, genetic; dna sequence; gene dosage; nucleic acid hybridization; polymorphism, genetic; dna polymorphism; gene replication; gene technology; dna hybridization; dna probes; humans; human; priority journal; article; dna probe |
| Journal Title: | Genome Research |
| Volume: | 10 |
| Issue: | 11 |
| ISSN: | 1088-9051 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Date Published: | 2000-11-01 |
| Start Page: | 1726 |
| End Page: | 1736 |
| Language: | English |
| DOI: | 10.1101/gr.138300 |
| PUBMED: | 11076858 |
| PROVIDER: | scopus |
| PMCID: | PMC310939 |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
