DNA microchips: Technical and practical considerations Journal Article
| Authors: | Sánchez-Carbayo, M.; Bornmann, W.; Cordon-Cardo, C. |
| Article Title: | DNA microchips: Technical and practical considerations |
| Abstract: | The development of high throughput techniques, such as DNA microarrays, engages interest in many biomedical research fields. They are becoming one of the preferred methods for large-scale expression analyses. The power of this technology is that it allows the profiling of thousands of genes in one single experiment. There are two main array-based technologies: cDNA and oligonucleotide arrays. cDNA arrays consist of microscope slides or nylon membranes containing hundreds to thousands of immobilized DNA probes, which are hybridized to fluorescent or radioactive complementary cDNA obtained from a target sample. Oligonucleotide chips differ in that probes are 20-25 mer selected oligonucleotides, which are bound to glass substrates and that the DNA obtained from a target sample can only be fluorescently labeled. In this review, we describe the different types of DNA-chips, the steps involved in the production of microchips, the methodological and technical aspects of microchip utilization, and their potential applications including some practical considerations utilizing clinical material. |
| Keywords: | gene sequence; sequence analysis; single nucleotide polymorphism; review; genetic analysis; gene expression; image analysis; genetic variability; genotype; rna; medical research; device; data analysis; dna microarray; molecular interaction; glass; diagnostic test; oligonucleotide; complementary dna; biomedical engineering; dna probe; nylon; molecular hybridization |
| Journal Title: | Current Organic Chemistry |
| Volume: | 4 |
| Issue: | 9 |
| ISSN: | 1385-2728 |
| Publisher: | Bentham Science Publishers |
| Date Published: | 2000-09-01 |
| Start Page: | 945 |
| End Page: | 971 |
| Language: | English |
| PROVIDER: | scopus |
| DOI: | 10.2174/1385272003375987 |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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