The use of pHluorins for optical measurements of presynaptic activity Journal Article
| Authors: | Sankaranarayanan, S.; De Angelis, D.; Rothman, J. E.; Ryan, T. A. |
| Article Title: | The use of pHluorins for optical measurements of presynaptic activity |
| Abstract: | Genetically encoded reporters for optical measurements of presynaptic activity hold significant promise for measurements of neurotransmission within intact or semi-intact neuronal networks. We have characterized pH-sensitive green fluorescent protein-based sensors (pHluorins) of synaptic vesicle cycling at nerve terminals. pHluorins have a pK ~ 7.1, which make them ideal for tracking synaptic vesicle lumen pH upon cycling through the plasma membrane during action potentials. A theoretical analysis of the expected signals using this approach and guidelines for future reporter development are provided. |
| Keywords: | nonhuman; animal cell; animal; ph; luminescent proteins; presynaptic terminals; rat; newborn; measurement; fluorescence microscopy; rats; rats, sprague-dawley; endocytosis; optics; hydrogen-ion concentration; in vitro; exocytosis; biophysics; hippocampus; models, neurological; synaptic transmission; action potentials; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Biophysical Journal |
| Volume: | 79 |
| Issue: | 4 |
| ISSN: | 0006-3495 |
| Publisher: | Cell Press |
| Date Published: | 2000-10-01 |
| Start Page: | 2199 |
| End Page: | 2208 |
| Language: | English |
| PUBMED: | 11023924 |
| PROVIDER: | scopus |
| PMCID: | PMC1301110 |
| DOI: | 10.1016/S0006-3495(00)76468-X |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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