Patterns of synaptic activity in neural networks recorded by light emission from synaptolucins Journal Article
| Authors: | Miesenböck, G.; Rothman, J. E. |
| Article Title: | Patterns of synaptic activity in neural networks recorded by light emission from synaptolucins |
| Abstract: | The emission of light, coupled to exocytosis, can in principle be utilized to monitor the activity of a large number of individual synapses simultaneously. To illustrate this concept, fusion proteins of Cypridina luciferase and synaptotagmin-I or VAMP-2/synaptobrevin (which we term 'synaptolucins') were expressed in cultured hippocampal neurons with the help of viral vectors. Synaptolucins were targeted to synaptic vesicles and, upon exocytosis, formed light-emitting complexes with their cognate luciferin, which was added to the extracellular medium. Photon emissions required a depolarizing stimulus, occurred from regions with high synaptic density as ascertained by vital staining of recycling synaptic vesicles, and were sensitive to Ca2+ depletion and clostridial neurotoxins. The method can currently detect exocytosis of the readily releasable pool of synaptic vesicles at a hippocampal synapse, corresponding to about two dozen quanta, but has the potential for greater sensitivity. |
| Keywords: | nonhuman; animals; animal tissue; gene expression; luciferase; nerve tissue proteins; membrane proteins; diagnostic imaging; animalia; molecular sequence data; hybrid protein; membrane glycoproteins; rat; rats; calcium-binding proteins; rats, sprague-dawley; luciferases; nerve cell network; synaptotagmin; photoactivation; nerve net; exocytosis; synapses; synapse; hippocampus; synaptotagmin i; light; optical imaging; neurotransmission; synaptic transmission; r-snare proteins; pc12 cells; synapse vesicle; priority journal; article; synaptotagmins; cyprinidae; cypridina |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 94 |
| Issue: | 7 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1997-04-01 |
| Start Page: | 3402 |
| End Page: | 3407 |
| Language: | English |
| DOI: | 10.1073/pnas.94.7.3402 |
| PUBMED: | 9096406 |
| PROVIDER: | scopus |
| PMCID: | PMC20382 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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