Comparative genomic hybridization study of de novo myeloid neoplasia Journal Article
| Authors: | Castuma, M. V.; Rao, P. H.; Acevedo, S. H.; Larripa, I. B. |
| Article Title: | Comparative genomic hybridization study of de novo myeloid neoplasia |
| Abstract: | Comparative genomic hybridization (CGH) was used to detect chromosomal imbalances in 20 patients with a diagnosis of myelodysplastic syndrome (MDS) and acute myeloid leukaemia (AML). The results obtained were compared with G-banding analysis. This last methodology showed 50% of cases with clonal abnormalities whereas CGH detected 70% of cases with copy number changes. Gains were more frequent than losses and constituted 66% of total changes detected. The most common gains included chromosomes 21 and chromosome region 18p for AML and chromosome 17 and region 1p33p35 for MDS. Losses represent 34% of changes and the regions involved were 5q31q32, 7q22, 7p12 and 13q21q22. CGH revealed additional chromosome imbalances in 12 of 20 cases (60%) which were not detected by traditional cytogenetic studies, demonstrating complex karyotype in 50% (6/12). Combination of CGH and G-banding provides an efficient method to identify critical regions present in the malignant clone, which is of great value in the prognosis and outcome of myeloid neoplasias. Copyright (C) 2000 S. Karger AG, Basel. |
| Keywords: | adolescent; adult; child; clinical article; controlled study; treatment outcome; aged; child, preschool; middle aged; acute granulocytic leukemia; diagnostic accuracy; in situ hybridization, fluorescence; cytogenetics; chromosome aberration; myelodysplastic syndrome; diagnostic value; leukemia, myeloid; intermethod comparison; chromosome aberrations; gene dosage; nucleic acid hybridization; karyotype; karyotyping; clone cells; cytogenetic analysis; gene location; myelodysplastic syndromes; acute disease; chromosome map; trisomy; monosomy; dna hybridization; chromosome banding; chromosome high resolution banding analysis; humans; prognosis; human; male; female; priority journal; article; acute myeloid leukaemia; chromosome imbalances; genomic hybridization, comparative |
| Journal Title: | Acta Haematologica |
| Volume: | 104 |
| Issue: | 1 |
| ISSN: | 0001-5792 |
| Publisher: | S. Karger AG |
| Date Published: | 2000-11-01 |
| Start Page: | 25 |
| End Page: | 30 |
| Language: | English |
| PUBMED: | 11111118 |
| PROVIDER: | scopus |
| DOI: | 10.1159/000041065 |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
