Frequent gain of chromosome 19 in megakaryoblastic leukemias detected by comparative genomic hybridization Journal Article
| Authors: | Alvarez, S.; Macgrogan, D.; Calasanz, M. J.; Nimer, S. D.; Jhanwar, S. C. |
| Article Title: | Frequent gain of chromosome 19 in megakaryoblastic leukemias detected by comparative genomic hybridization |
| Abstract: | Acute megakaryocytic leukemia is a rare subtype of AML that is often difficult to diagnose; it is most commonly associated with Down syndrome in children. To identify chromosomal imbalances and rearrangements associated with acute megakaryocytic leukemia, we used G-banding, comparative genomic hybridization (CGH), and whole chromosome painting (WCP) on a variety of primary patients' samples and leukemia cell lines. The most common abnormality was gain of chromosome 19 or arm 19q, which was detected by CGH in four of 12 (33.3%) primary samples and nine of 11 (81.8%) cell lines. In none of the primary samples was this abnormality detected by G-banding analysis. WCP was used to define further the nature of the chromosome 19 gain in the cell lines, which was found to be due to the presence of additional 19q material on marker chromosomes or to cryptic translocations involving 19q. The most common chromosomal loss-detected only in the cell lines-was deletion of chromosomal band 13q14, which was seen in six of 11 (54.5%) cell lines. Other recurrent changes included gains of 1p, 6p, 8q, 11q, 15q, 17q, and 21q and losses of 2, 4q, 5q, 7q, 9p, and 11p. Combining conventional and molecular cytogenetic analyses defined recurrent clonal chromosomal abnormalities, which will aid in the identification of critical genes that are abnormal in acute megakaryocytic leukemia cells. © 2001 Wiley-Liss, Inc. |
| Keywords: | adult; child; clinical article; aged; child, preschool; middle aged; human cell; chromosome 19; gene amplification; cell line; cytogenetics; chromosome aberration; chromosome translocation; chromosomes, human, pair 19; chromosome aberrations; nucleic acid hybridization; chromosome loss; comparative genomic hybridization; chromosome painting; acute megakaryocytic leukemia; leukemia, megakaryocytic, acute; chromosome banding pattern; chromosome deletion 13; humans; human; male; female; priority journal; article; megakaryoblast |
| Journal Title: | Genes Chromosomes and Cancer |
| Volume: | 32 |
| Issue: | 3 |
| ISSN: | 1045-2257 |
| Publisher: | Wiley Periodicals, Inc |
| Date Published: | 2001-11-01 |
| Start Page: | 285 |
| End Page: | 293 |
| Language: | English |
| DOI: | 10.1002/gcc.1192 |
| PUBMED: | 11579469 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
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