Frequent amplification and rearrangement of chromosomal bands 6p12-p21 and 17p11.2 in osteosarcoma Journal Article
| Authors: | Lau, C. C.; Harris, C. P.; Lu, X. Y.; Perlaky, L.; Gogineni, S.; Chintagumpala, M.; Hicks, J.; Johnson, M. E.; Davino, N. A.; Huvos, A. G.; Meyers, P. A.; Healey, J. H.; Gorlick, R.; Rao, P. H. |
| Article Title: | Frequent amplification and rearrangement of chromosomal bands 6p12-p21 and 17p11.2 in osteosarcoma |
| Abstract: | Osteosarcoma (OS) is a highly malignant bone neoplasm of children and young adults. It is characterized by chaotic karyotypes with complex marker chromosomes. We applied a combination of molecular cytogenetic techniques including comparative genomic hybridization (CGH), spectral karyotyping (SKY), and fluorescence in situ hybridization (FISH) to decipher the chromosomal complexity in a panel of 25 tumors. Combined SKY and G-banding analysis identified several novel recurrent breakpoint clusters and 9 nonrecurrent reciprocal translocations. CGH identified several recurrent chromosomal losses including 2q, 3p, 9, 10p, 12q, 13q, 14q, 15q, 16, 17p, and 18q, gains including Xp, Xq, 5q, 6p, 8q, 17p, and 20q, and high-level chromosomal amplifications at Xp11.2, 1q21-q22, 4p11, 4q12, 5p15, 6p12.1, 8q13, 8q23, 10q11, 10q22, 11q13, 11q23, 12q13-q14, 13q21-q34, 16q22, 17p11.2, 17q21-q22, 18q22, 20p11.2, and 20q12. Frequent amplification and rearrangement involving chromosomal bands at 6p12-p21 and 17p11.2 were found in 28% and 32% of cases, respectively. In an attempt to identify the genes involved in these amplicons, we used three nonoverlapping BAC clones contained within each amplicon as probes for FISH analysis, leading to a more detailed characterization and quantification of the 6p and 17p amplicons. © 2003 Wiley-Liss, Inc. |
| Keywords: | osteosarcoma; adolescent; adult; child; clinical article; controlled study; human tissue; school child; gene cluster; gene translocation; human cell; in situ hybridization, fluorescence; gene amplification; molecular dynamics; gene frequency; cytogenetics; molecular cloning; fluorescence in situ hybridization; gene rearrangement; gene identification; amplicon; chromosome breakage; chromosomes, human, pair 6; gene dosage; nucleic acid hybridization; karyotype; comparative genomic hybridization; karyotyping; molecular probe; chromosome painting; chromosomes, human, pair 17; chromosome banding; chromosome banding pattern; humans; human; male; female; priority journal; article |
| Journal Title: | Genes Chromosomes and Cancer |
| Volume: | 39 |
| Issue: | 1 |
| ISSN: | 1045-2257 |
| Publisher: | Wiley Periodicals, Inc |
| Date Published: | 2004-01-01 |
| Start Page: | 11 |
| End Page: | 21 |
| Language: | English |
| DOI: | 10.1002/gcc.10291 |
| PROVIDER: | scopus |
| PUBMED: | 14603437 |
| DOI/URL: | |
| Notes: | Genes Chromosomes Cancer -- Cited By (since 1996):77 -- Export Date: 16 June 2014 -- CODEN: GCCAE -- Source: Scopus |
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