Evaluation of new automated hematopoietic progenitor cell analysis in the clinical management of peripheral blood stem cell collections Journal Article
| Authors: | Peerschke, E. I.; Moung, C.; Pessin, M. S.; Maslak, P. |
| Article Title: | Evaluation of new automated hematopoietic progenitor cell analysis in the clinical management of peripheral blood stem cell collections |
| Abstract: | Background Successful peripheral blood stem cell transplantation (PBSCT) depends on the collection and infusion of adequate numbers of peripheral blood progenitor cells (PBPCs). Several predictors of PBPC yield are used currently, including white blood cell (WBC) count and CD34 analysis. This study evaluated the utility of the new automated hematopoietic progenitor cell count available on Sysmex XN hematology analyzers (XN-HPCs) in PBSCT. Study Design and Methods The performance characteristics of XN-HPC, CD34+, and WBC analysis were compared using 107 matched peripheral blood and apheresis samples. Results Good correlation was observed between XN-HPC and CD34+ cell counts in peripheral blood (r-‰=-‰0.88; slope, 0.81) and apheresis collections (r-‰=-‰0.91; slope, 0.89). Moreover, peripheral blood XN-HPC and CD34 analysis showed comparable ability to predict successful PBPC harvests (≥2 × 106 CD34+ cells/kg). At a cutoff of 20 × 106 progenitor cells/L, peripheral blood XN- HPC and CD34 analysis both showed negative predictive values (NPVs) of 100% and positive predictive values (PPVs) of 55.4 and 63%, respectively. Using an optimized cutoff of 38 × 106 progenitor cells/L, derived from receiver operating characteristic analysis, the PPV for XN-HPC and CD34 analysis increased to 71.4 and 78.9%, respectively, with relatively unchanged NPVs (XN-HPC 97.7%, CD34+ 98.0%). In contrast, the correlation between peripheral blood WBC and CD34 analysis was poor (r-‰=-‰0.48; slope, 669.85), and the peripheral blood WBC count (cutoff, 10 × 109/L) was a poor predictor of PBPC harvest (NPV 60%, PPV 43.1%). Conclusion XN-HPC compares favorably with CD34 analysis and may be a surrogate for CD34 analysis to predict optimal timing of PBPC collections. © 2015 AABB. |
| Keywords: | adolescent; adult; aged; human cell; blood sampling; peripheral blood stem cell; stem cell mobilization; hematopoietic stem cell; leukocyte count; granulocyte colony stimulating factor; plerixafor; cd34 selection; leukocyte; predictive value; apheresis; procedures concerning cells; hematology analyzer; human; male; female; article; automated hematopoietic progenitor cell analysis; hematological procedure |
| Journal Title: | Transfusion |
| Volume: | 55 |
| Issue: | 8 |
| ISSN: | 0041-1132 |
| Publisher: | Blackwell Publishing |
| Date Published: | 2015-08-01 |
| Start Page: | 2001 |
| End Page: | 2009 |
| Language: | English |
| DOI: | 10.1111/trf.13078 |
| PROVIDER: | scopus |
| PUBMED: | 25808236 |
| PMCID: | PMC4976989 |
| DOI/URL: | |
| Notes: | Export Date: 2 September 2015 -- Source: Scopus |
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