Characterization of banked umbilical cord blood hematopoietic progenitor cells and lymphocyte subsets and correlation with ethnicity, birth weight, sex, and type of delivery: A Cord Blood Transplantation (COBLT) Study report Journal Article

  

Authors:	Cairo, M. S.; Wagner, E. L.; Fraser, J.; Cohen, G.; van de Ven, C.; Carter, S. L.; Kernan, N. A.; Kurtzberg, J.
Article Title:	Characterization of banked umbilical cord blood hematopoietic progenitor cells and lymphocyte subsets and correlation with ethnicity, birth weight, sex, and type of delivery: A Cord Blood Transplantation (COBLT) Study report
Title Series:	33rd Annual Meeting of the International-Society-for-Experimental-Hematology
Abstract:	Background: The Cord Blood Transplantation (COBLT) Study banking program was initiated in 1996. The study goals were to develop standard operating procedures for cord blood (CB) donor recruitment and banking and to build an ethnically diverse unrelated CB bank to support a transplantation protocol. Study Design and Methods: The hematopoietic progenitor cell (HPC) and lymphocyte subset (LS) content of approximately 8000 CB units were characterized, and these results were correlated with donor ethnicity, birth weight, gestational age, sex, and type of delivery. Results: There was a significant correlation of CD34+ cell count with colony-forming unit (CFU)-granulocyte-macrophage (r=0.68, p < 0.001), CFU-granulocyte-erythroid-macrophage-megakaryocyte (r=0.52, p < 0.001), burst-forming unit-erythroid (BFU-E; r=0.61, p < 0.001), and total CFUs (r=0.67, p < 0.001). Nucleated red blood cell count was significantly correlated with total CD34+ (r=0.56, p < 0.001), total CFU (r=0.50, p < 0.001), BFU-E (r=0.48, p < 0.001), and counts of CD34+ subsets (p < 0.001). Caucasian ethnicity was significantly correlated with higher CD3+/CD4+, CD19+, and CD16+/CD56+ LSs. Furthermore, CD34+/CD38- and CD34+/CD61+ CB units (HPC-C) were significantly lower in African American and Asian persons compared to Caucasian and Hispanic persons. Male sex was associated with significantly fewer CD3+/CD4+, CD19+, and CD16+/CD56+ but increased CD3+/CD8+ LSs (p < 0.001). Finally, cesarean section was associated with significantly higher total CFU and CD16+/CD56+ but lower CD3+/CD4+, CD3+/CD8+, and CD19+ LSs. Conclusion: These results provide a standard and range for uniformly processed HPC-C progenitor cells and LSs. CB progenitor cells and/or LSs may in the future predict for rapidity of engraftment, incidence of graft-versus-host disease, speed and quality of immunore-constitution, graft-versus-tumor effects, and/or success of gene transfection after CB transplantation.
Keywords:	engraftment; versus-host-disease; stem-cells; mononuclear-cells; recipients; unrelated donors; placental-blood; bone-marrow reconstitution; nonmalignant disease; activated cord
Journal Title:	Transfusion
Volume:	45
Issue:	6
Dates:	JUL 17-20, 2004
ISSN:	0041-1132
Publisher:	Blackwell Publishing
Publication Place:	New Orleans, LA
Date Published:	2005-06-01
Start Page:	856
End Page:	866
Language:	English
DOI:	10.1111/j.1537-2995.2005.04429.x
ACCESSION:	WOS:000229224400006
PROVIDER:	wos
PUBMED:	15934982
Notes:	--- - Article; Proceedings Paper - 33rd Annual Meeting of the International-Society-for-Experimental-Hematology - JUL 17-20, 2004 - New Orleans, LA - "Source: Wos"

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