The effect of the composition of unrelated donor bone marrow and peripheral blood progenitor cell grafts on transplantation outcomes Journal Article
| Authors: | Collins, N. H.; Gee, A. P.; Durett, A. G.; Kan, F.; Zhang, M. J.; Champlin, R. E.; Confer, D.; Eapen, M.; Howard, A.; King, R.; Laughlin, M. J.; Plante, R. J.; Setterholm, M.; Spellman, S.; Keever-Taylor, C.; Wagner, J. E.; Weisdorf, D. J. |
| Article Title: | The effect of the composition of unrelated donor bone marrow and peripheral blood progenitor cell grafts on transplantation outcomes |
| Abstract: | To test the hypothesis that the outcome of hematopoietic stem cell (HSC) grafts is at least partially determined by the cellular composition of the graft, the National Marrow Donor Program (NMDP) analyzed the correlation of cellular phenotypes of unrelated grafts with graft outcome. Samples from 94 bone marrow (BM) and 181 peripheral blood progenitor cell (PBPC) grafts for transplantations at 40 U.S. transplant centers between 2003 and 2005 were analyzed at a single immunophenotyping reference laboratory. Samples were shipped from transplant centers upon receipt of graft. Graft cellular composition included analysis of leukocyte total cell numbers, and subsets of myeloid [CD34+, CD34+ CD38-], lymphoid [CD3+, CD3+ CD4+, CD3+ CD8+], and activated lymphoid cells [CD3+ CD25+, CD3+ CD69+, CD3+ HLA-DR+] coexpressing CD3+. There was substantial variability in the cellular composition of BM and PBPC grafts before and after graft processing by red blood cell (RBC) removal or plasma depletion in preparation for transplant. With BM grafts, cellular composition was not associated with hematopoietic recovery, graft-versus-host disease (GVHD), or survival. With PBPC grafts, survival rates were higher with CD34+ > 5 × 106/kg, 59% compared to 34% with CD34+ ≤ 5 × 106/kg at 1 year. Platelet recovery was higher with PBPC containing CD3+ CD8+ >8 × 107/kg. Neutrophil recovery or GVHD could not be predicted by any cellular subsets of PBPC grafts. Although survival was superior with PBPC grafts containing >5 × 106 CD34+/kg, an optimal graft mix of myeloid, lymphoid, and activated lymphoid subsets was not identified. © 2010 American Society for Blood and Marrow Transplantation. |
| Keywords: | adolescent; adult; child; controlled study; school child; treatment outcome; survival analysis; major clinical study; overall survival; flow cytometry; cd8+ t lymphocyte; reproducibility of results; phenotype; cd34 antigen; hematopoietic stem cell transplantation; acute graft versus host disease; chronic graft versus host disease; registries; peripheral blood stem cell; hla dr antigen; immunophenotyping; bone marrow cell; leukocyte count; graft survival; antigens, cd; cd25+ t lymphocyte; cd3+ t lymphocyte; bone marrow transplantation; donor selection; graft vs host disease; peripheral blood stem cell transplantation; graft recipient; cd38 antigen; myeloid cells; lymphoid cell; organ donor; cd69 antigen; graft composition; hematopoietic recovery; unrelated donor transplant; cell composition; lymphocyte subsets |
| Journal Title: | Biology of Blood and Marrow Transplantation |
| Volume: | 16 |
| Issue: | 2 |
| ISSN: | 1083-8791 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2010-02-01 |
| Start Page: | 253 |
| End Page: | 262 |
| Language: | English |
| DOI: | 10.1016/j.bbmt.2009.10.004 |
| PUBMED: | 19822219 |
| PROVIDER: | scopus |
| PMCID: | PMC2952392 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 2" - "Export Date: 20 April 2011" - "CODEN: BBMTF" - "Source: Scopus" |
