Interaction of a transcriptional repressor with the RNA polymerase II holoenzyme plays a crucial role in repression Journal Article
| Authors: | Zaman, Z.; Ansari, A. Z.; Koh, S. S.; Young, R.; Ptashne, M. |
| Article Title: | Interaction of a transcriptional repressor with the RNA polymerase II holoenzyme plays a crucial role in repression |
| Abstract: | The yeast transcriptional repressor Tup1, tethered to DNA, represses to strikingly different degrees transcription elicited by members of two classes of activators. Repression in both cases is virtually eliminated by mutation of either member of the cyclinkinase pair Srb10/11. In contrast, telomeric chromatin affects both classes of activators equally, and in neither case is that repression affected by mutation of Srb10/11. In vitro, Tup1 interacts with RNA polymerase II holoenzyme bearing Srb10 as well as with the separated Srb10. These and other findings indicate that at least one aspect of Tup1's action involves interaction with the RNA polymerase II holoenzyme. |
| Keywords: | mutation; nonhuman; telomere; protein binding; nuclear proteins; dna; transcription regulation; chromatin; gene repression; gene control; molecular interaction; yeast; saccharomyces cerevisiae proteins; cyclin-dependent kinases; repressor proteins; rna polymerase ii; fungal proteins; promoter regions (genetics); priority journal; article |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 98 |
| Issue: | 5 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2001-02-27 |
| Start Page: | 2550 |
| End Page: | 2554 |
| Language: | English |
| DOI: | 10.1073/pnas.041611198 |
| PUBMED: | 11226276 |
| PROVIDER: | scopus |
| PMCID: | PMC30175 |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
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