Interaction of a transcriptional repressor with the RNA polymerase II holoenzyme plays a crucial role in repression Journal Article


Authors: Zaman, Z.; Ansari, A. Z.; Koh, S. S.; Young, R.; Ptashne, M.
Article Title: Interaction of a transcriptional repressor with the RNA polymerase II holoenzyme plays a crucial role in repression
Abstract: The yeast transcriptional repressor Tup1, tethered to DNA, represses to strikingly different degrees transcription elicited by members of two classes of activators. Repression in both cases is virtually eliminated by mutation of either member of the cyclinkinase pair Srb10/11. In contrast, telomeric chromatin affects both classes of activators equally, and in neither case is that repression affected by mutation of Srb10/11. In vitro, Tup1 interacts with RNA polymerase II holoenzyme bearing Srb10 as well as with the separated Srb10. These and other findings indicate that at least one aspect of Tup1's action involves interaction with the RNA polymerase II holoenzyme.
Keywords: mutation; nonhuman; telomere; protein binding; nuclear proteins; dna; transcription regulation; chromatin; gene repression; gene control; molecular interaction; yeast; saccharomyces cerevisiae proteins; cyclin-dependent kinases; repressor proteins; rna polymerase ii; fungal proteins; promoter regions (genetics); priority journal; article
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 98
Issue: 5
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 2001-02-27
Start Page: 2550
End Page: 2554
Language: English
DOI: 10.1073/pnas.041611198
PUBMED: 11226276
PROVIDER: scopus
PMCID: PMC30175
DOI/URL:
Notes: Export Date: 21 May 2015 -- Source: Scopus
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  1. Mark Ptashne
    61 Ptashne
  2. Aseem Z Ansari
    10 Ansari
  3. Zafar Zaman
    6 Zaman