Machine-based method for multiplex in situ molecular characterization of tissues by immunofluorescence detection Journal Article
| Authors: | Yarilin, D.; Xu, K.; Turkekul, M.; Fan, N.; Romin, Y.; Fijisawa, S.; Barlas, A.; Manova-Todorova, K. |
| Article Title: | Machine-based method for multiplex in situ molecular characterization of tissues by immunofluorescence detection |
| Abstract: | Immunofluorescent staining is an informative tool that is widely used in basic research. Automation of immunostaining improves reproducibility and quality of the results. Up to now, use of automation in immunofluorescent staining was mostly limited to one marker. Here we present tyramide signal amplification based method of multiple marker immunofluorescent detection, including detection of antibodies, raised in the same species, in tissue sections and cultured cells. This method can be beneficial for both basic and clinical research. © 2015 Macmillan Publishers Limited. All rights reserved. |
| Journal Title: | Scientific Reports |
| Volume: | 5 |
| ISSN: | 2045-2322 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2015-03-31 |
| Start Page: | 9534 |
| Language: | English |
| DOI: | 10.1038/srep09534 |
| PROVIDER: | scopus |
| PUBMED: | 25826597 |
| PMCID: | PMC4821037 |
| DOI/URL: | |
| Notes: | Export Date: 4 May 2015 -- Source: Scopus |
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