Automated double in situ detection of mouse Lgr5 mRNA and lysozyme protein in examining the neighboring cell types of the mouse intestinal crypt Journal Article

  

Authors:	Turkekul, M.; Barlas, A.; Yarilin, D.; Fujisawa, S.; Fan, N.; Brendel, M.; Manova-Todorova, K.
Article Title:	Automated double in situ detection of mouse Lgr5 mRNA and lysozyme protein in examining the neighboring cell types of the mouse intestinal crypt
Abstract:	Automated detection of mRNAs and proteins in the same tissue sections is not a routine procedure. Successful experiment depends on the preparation of the tissue, the detection procedure, as well as the quality of the probes and antibodies. The multiplexed detections require experimental conditions, preserving the state of the molecular targets of interest and providing expression pattern of each target the same as in a single detection. Here we describe in detail the automated protocols used to detect mouse Lgr5 mRNA by in situ hybridization and immunofluorescence detection of lysozyme in the same mouse intestinal sections. Both the in situ hybridization and the protein detection were performed with an automated staining processor and provided strong and reproducible results. © Springer Science+Business Media LLC 2017.
Keywords:	stem cells; lysozyme; colorimetric and fluorescence mrna detection; immunofluorescence (if); intestinal crypt; leica bond rx; mlgr5 mrna in situ hybridization; mouse intestine; rna scope 2.5 ls
Journal Title:	Methods in Molecular Biology
Volume:	1554
ISSN:	1064-3745
Publisher:	Humana Press Inc
Date Published:	2017-01-01
Start Page:	263
End Page:	272
Language:	English
DOI:	10.1007/978-1-4939-6759-9_19
PROVIDER:	scopus
PUBMED:	28185199
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013018691&doi=10.1007%2f978-1-4939-6759-9_19&partnerID=40&md5=700df031a65e2e51e9bf8e664650e952
Notes:	Book Chapter 19 in "Signal Transduction Immunohistochemistry: Methods and Protocols. 2nd ed" (ISBN: 978-1-4939-6757-5) -- Export Date: 2 March 2017 -- Source: Scopus

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