Validation of anti-mouse PDL-1 goat polyclonal antibody staining with mouse PDL-1 in situ hybridization on adjacent sections of cell pellets and mouse tumors Journal Article

   

Authors:	Ricca, J.; Turkekul, M.; Barlas, A.; Yarilin, D.; Fujisawa, S.; Fan, N.; Brendel, M.; Zamarin, D.; Wolchok, J. D.; Manova-Todorova, K.
Article Title:	Validation of anti-mouse PDL-1 goat polyclonal antibody staining with mouse PDL-1 in situ hybridization on adjacent sections of cell pellets and mouse tumors
Abstract:	Finding a valid antibody to detect mouse programmed death ligand 1 (PDL-1) by immunohistochemistry or immunofluorescence staining has been notoriously difficult. Successful validation of an antibody requires the use of multiple detection methods with the ability to compare appropriate positive and negative controls. Here, we describe in detail the protocols used to validate a mouse-specific PDL-1 antibody used in immunohistochemistry staining with an mRNA in situ hybridization on adjacent sections of mouse B16 tumor. This validation is supported by immunohistochemistry staining of PDL-1 on B16 cell pellets either treated or not treated with IFN-gamma. © Springer Science+Business Media LLC 2017.
Keywords:	in situ hybridization; interferon gamma; antibody validation; b16 melanoma cells; b16 mouse melanoma tumors; lieca bond rx; pdl-1; pdl-1 mrna; viewrna ish
Journal Title:	Methods in Molecular Biology
Volume:	1554
ISSN:	1064-3745
Publisher:	Humana Press Inc
Date Published:	2017-01-01
Start Page:	253
End Page:	262
Language:	English
DOI:	10.1007/978-1-4939-6759-9_18
PROVIDER:	scopus
PUBMED:	28185198
PMCID:	PMC5751444
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85012986105&doi=10.1007%2f978-1-4939-6759-9_18&partnerID=40&md5=dac3eef3fe53d138a8d3537466f3100c
Notes:	Book Chapter 18 in "Signal Transduction Immunohistochemistry: Methods and Protocols. 2nd ed" (ISBN: 978-1-4939-6757-5) -- Export Date: 2 March 2017 -- Source: Scopus

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