Authors: | Nie, S.; Tian, B.; Wang, X.; Pincus, D. H.; Welker, M.; Gilhuley, K.; Lu, X.; Han, Y. W.; Tang, Y. W. |
Article Title: | Fusobacterium nucleatum subspecies identification by matrix-assisted laser desorption ionization - Time of flight mass spectrometry |
Abstract: | We explored the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification of Fusobacterium nucleatum subspecies. MALDI-TOF MS spectra of five F. nucleatum subspecies (animalis, fusiforme, nucleatum, polymorphum, and vincentii) were analyzed and divided into four distinct clusters, including subsp. animalis, nucleatum, polymorphum, and fusiforme/vincentii. MALDI-TOF MS with the modified SARAMIS database further correctly identified 28 of 34 F. nucleatum clinical isolates to the subspecies level. Copyright © 2015, American Society for Microbiology. All Rights Reserved. |
Keywords: | fusobacterium nucleatum |
Journal Title: | Journal of Clinical Microbiology |
Volume: | 53 |
Issue: | 4 |
ISSN: | 0095-1137 |
Publisher: | American Society for Microbiology |
Date Published: | 2015-04-01 |
Start Page: | 1399 |
End Page: | 1402 |
Language: | English |
DOI: | 10.1128/jcm.00239-15 |
PROVIDER: | scopus |
PUBMED: | 25653408 |
PMCID: | PMC4365237 |
DOI/URL: | |
Notes: | Export Date: 2 April 2015 -- Source: Scopus |