MALDI-TOF mass spectrometry distinguishes daratumumab from M-proteins Journal Article
| Authors: | Moore, L. M.; Cho, S.; Thoren, K. L. |
| Article Title: | MALDI-TOF mass spectrometry distinguishes daratumumab from M-proteins |
| Abstract: | Background: Daratumumab, a therapeutic IgG kappa monoclonal antibody, can cause a false positive interference on electrophoretic assays that are routinely used to monitor patients with monoclonal gammopathies. In this study, we evaluate the ability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to distinguish daratumumab from disease-related IgG kappa monoclonal proteins (M-protein). Methods: Waste clinical samples from 31 patients who were receiving daratumumab and had a history of IgG kappa monoclonal gammopathy were collected. Immunoglobulins were purified from serum and analyzed by MALDI-TOF MS. Mass spectra were assessed for the presence of distinct monoclonal proteins. For samples in which only one monoclonal peak was identified near the expected m/z of daratumumab, the Hydrashift 2/4 Daratumumab Assay was used to confirm the presence of an M-protein. Results: Using MALDI-TOF MS, daratumumab could be distinguished from M-proteins in 26 out of 31 samples (84%). Results from 2 samples were inconclusive since the M-protein was not detected by the Hydrashift assay and may also be undetectable by MALDI-TOF MS. Comparatively, daratumumab was distinguishable from M-proteins in 14 out of 31 samples (45%) by immunofixation. Conclusions: MALDI-TOF MS offers greater specificity compared to immunofixation for distinguishing daratumumab from M-proteins. © 2019 |
| Keywords: | multiple myeloma; maldi-tof mass spectrometry; daratumumab; monoclonal immunoglobulin |
| Journal Title: | Clinica Chimica Acta |
| Volume: | 492 |
| ISSN: | 0009-8981 |
| Publisher: | Elsevier Science BV |
| Date Published: | 2019-05-01 |
| Start Page: | 91 |
| End Page: | 94 |
| Language: | English |
| DOI: | 10.1016/j.cca.2019.02.017 |
| PROVIDER: | scopus |
| PUBMED: | 30790545 |
| PMCID: | PMC6524149 |
| DOI/URL: | |
| Notes: | Source: Scopus |
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