Identification of gamma heavy chain disease using MALDI-TOF mass spectrometry Journal Article


Authors: Thoren, K. L.; Eveillard, M.; Chan, P.; Doddi, S.; Cho, S.; Murata, K.
Article Title: Identification of gamma heavy chain disease using MALDI-TOF mass spectrometry
Abstract: We describe the use of MALDI-TOF mass spectrometry in the analysis of a suspected case of gamma heavy chain disease. The patient had an abnormal serum immunofixation result where a monoclonal gamma heavy chain band was present without a corresponding light chain. Analysis by MALDI-TOF mass spectrometry revealed large peaks in the spectrum following IgG-specific purification. The m/z values of the peaks were outside the expected range for normal heavy chains or light chains. Corresponding peaks were not present in mass spectra of the kappa- or lambda-specific purifications. MALDI-TOF MS confirmed the presence of a truncated heavy chain without associated light chains. This case report demonstrates the value of mass spectrometry in interpreting challenging cases such as the identification of heavy chain disease. © 2019 The Canadian Society of Clinical Chemists
Keywords: maldi-tof mass spectrometry; monoclonal immunoglobulin; heavy chain disease
Journal Title: Clinical Biochemistry
Volume: 77
ISSN: 0009-9120
Publisher: Elsevier Inc.  
Date Published: 2020-03-01
Start Page: 57
End Page: 61
Language: English
DOI: 10.1016/j.clinbiochem.2019.12.010
PUBMED: 31884198
PROVIDER: scopus
PMCID: PMC7046309
DOI/URL:
Notes: Article -- Export Date: 2 March 2020 -- Source: Scopus
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MSK Authors
  1. Kazunori Murata
    21 Murata
  2. Katie Lynn Thoren
    30 Thoren
  3. Sun Min Cho
    9 Cho
  4. Sital Doddi
    1 Doddi
  5. Patrick Chan
    1 Chan