Whole-genome analysis of muscle founder cells implicates the chromatin regulator Sin3A in muscle identity Journal Article

   

Authors:	Dobi, K. C.; Halfon, M. S.; Baylies, M. K.
Article Title:	Whole-genome analysis of muscle founder cells implicates the chromatin regulator Sin3A in muscle identity
Abstract:	Skeletal muscles are formed in numerous shapes and sizes, and this diversity impacts function and disease susceptibility. To understand how muscle diversity is generated, we performed gene expression profiling of two muscle subsets from Drosophila embryos. By comparing the transcriptional profiles of these subsets, we identified a core group of founder cell-enriched genes. We screened mutants for muscle defects and identified functions for Sin3A and 10 other transcription and chromatin regulators in the Drosophila embryonic somatic musculature. Sin3A is required for the morphogenesis of a muscle subset, and Sin3A mutants display muscle loss and misattachment. Additionally, misexpression of identity gene transcription factors in Sin3A heterozygous embryos leads to direct transformations of one muscle into another, whereas overexpression of Sin3A results in the reverse transformation. Our data implicate Sin3A as a key buffer controlling muscle responsiveness to transcription factors in the formation of muscle identity, thereby generating tissue diversity. © 2014 The Authors.
Keywords:	controlled study; nonhuman; animal cell; somatic cell; gene overexpression; gene expression profiling; embryo; genetic variability; drosophila; genetic transcription; genome analysis; microarray analysis; cell transformation; gene identification; muscle disease; upregulation; skeletal muscle; muscle cell; muscle development; developmental gene; transcription factor sin3a; priority journal; article; alh gene; chn gene; crp gene; elo b gene; gug gene; kdm2 gene; lid gene; lola gene; med13 gene; nom gene; sin3a gene
Journal Title:	Cell Reports
Volume:	8
Issue:	3
ISSN:	2211-1247
Publisher:	Cell Press
Date Published:	2014-08-07
Start Page:	858
End Page:	870
Language:	English
DOI:	10.1016/j.celrep.2014.07.005
PROVIDER:	scopus
PMCID:	PMC4207094
PUBMED:	25088419
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84924574417&partnerID=40&md5=6a7ade413f350ca0b556565eceb6e18a
Notes:	Export Date: 2 April 2015 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/70740