Inflammatory monocytes facilitate adaptive CD4 T cell responses during respiratory fungal infection Journal Article

   

Authors:	Hohl, T. M.; Rivera, A.; Lipuma, L.; Gallegos, A.; Shi, C.; Mack, M.; Pamer, E. G.
Article Title:	Inflammatory monocytes facilitate adaptive CD4 T cell responses during respiratory fungal infection
Abstract:	Aspergillus fumigatus, a ubiquitous fungus, causes invasive disease in immunocompromised humans. Although monocytes and antigen-specific CD4 T cells contribute to defense against inhaled fungal spores, how these cells interact during infection remains undefined. Investigating the role of inflammatory monocytes and monocyte-derived dendritic cells during fungal infection, we find that A. fumigatus infection induces an influx of chemokine receptor CCR2- and Ly6C-expressing inflammatory monocytes into lungs and draining lymph nodes. Depletion of CCR2<sup>+</sup> cells reduced A. fumigatus conidial transport from lungs to draining lymph nodes, abolished CD4 T cell priming following respiratory challenge, and impaired pulmonary fungal clearance. In contrast, depletion of CCR2<sup>+</sup>Ly6C<sup>hi</sup> monocytes during systemic fungal infection did not prevent CD4 T cell priming in the spleen. Our findings demonstrate that pulmonary CD4 T cell responses to inhaled spores require CCR2<sup>+</sup>Ly6C<sup>hi</sup> monocytes and their derivatives, revealing a compartmentally restricted function for these cells in adaptive respiratory immune responses. © 2009 Elsevier Inc. All rights reserved.
Keywords:	controlled study; nonhuman; flow cytometry; lymph nodes; animal cell; mouse; animals; mice; animal tissue; spleen; dendritic cell; animal experiment; animal model; inflammation; in vitro study; antigen presentation; dendritic cells; microbio; cellular immunity; lymph node; cd4+ t lymphocyte; cd4-positive t-lymphocytes; lung; cell movement; adoptive transfer; monocyte; monocytes; adaptive immunity; molimmuno; aspergillus fumigatus; fungi; chemokine receptor ccr2; aspergillosis; fungus spore; lung aspergillosis; respiratory tract infection; antigens, ly; pulmonary aspergillosis; receptors, ccr2
Journal Title:	Cell Host & Microbe
Volume:	6
Issue:	5
ISSN:	1931-3128
Publisher:	Cell Press
Date Published:	2009-11-19
Start Page:	470
End Page:	481
Language:	English
DOI:	10.1016/j.chom.2009.10.007
PUBMED:	19917501
PROVIDER:	scopus
PMCID:	PMC2785497
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-71749100858&partnerID=40&md5=3289c0eb45afb712bff41f98663cc323
Notes:	--- - "Cited By (since 1996): 3" - "Export Date: 30 November 2010" - "Source: Scopus"

https://synapse.mskcc.org/synapse/works/has_related_data_chk/685