SNAREs and associated regulators in the control of exocytosis in the RBL-2H3 mast cell line Journal Article
| Authors: | Blank, U.; Cyprien, B.; Martin-Verdeaux, S.; Paumet, F.; Pombo, I.; Rivera, J.; Roa, M.; Varin-Blank, N. |
| Article Title: | SNAREs and associated regulators in the control of exocytosis in the RBL-2H3 mast cell line |
| Abstract: | Mast cells participate in inflammation and allergies by releasing biologically active mediators stored in numerous cytoplasmic granules. Degranulation is tightly controlled and requires activation of cell surface receptors, such as the high affinity IgE receptor (FcεRI). Here, we discuss some of the key components of the molecular machinery that regulates the final steps of fusion between the granular and plasma membrane based on results obtained with the rat mast cell line RBL-2H3. We emphasize the role of soluble N-ethylmaleimide attachment protein receptors (SNAREs) proteins such as syntaxin 4 that can promote membrane fusion through formation of a stable complex with SNAP-23. We also highlight the role of a Ser/Thr kinase found to be associated with Rab3D, a negative regulator of degranulation. Associated kinase activity, which diminishes after stimulation as a consequence of intracellular calcium increases, specifically phosphorylates syntaxin 4 thereby affecting its capacity to bind to its t-SNARE partner SNAP-23. Our results suggest a new way of how Rab3 GTPases may intersect with the function of SNAREs thought to be universal mediators of membrane fusion. © 2002 Elsevier Science Ltd. All rights reserved. |
| Keywords: | protein phosphorylation; unclassified drug; review; nonhuman; conference paper; protein function; animal; animals; complex formation; biological model; models, biological; cell line; membrane proteins; calcium; tumor cells, cultured; enzyme activity; protein serine threonine kinase; physiology; immunology; cell culture; protein-serine-threonine kinases; membrane protein; rat; cell membrane; rats; molecular biology; isoprotein; calcium cell level; exocytosis; degranulation; vesicular transport proteins; guanosine triphosphatase; mast cell; mast cells; vesicular transport protein; membrane fusion; snare protein; syntaxin; qa-snare proteins; rab protein; cell granule; snare proteins; cell degranulation; human; priority journal; rab3d; rab3d protein; synaptosomal associated protein 23; rab3d protein, rat; rak3d protein, rat; rab3 gtp-binding proteins |
| Journal Title: | Molecular Immunology |
| Volume: | 38 |
| Issue: | 16-18 |
| ISSN: | 0161-5890 |
| Publisher: | Pergamon-Elsevier Science Ltd |
| Date Published: | 2002-09-01 |
| Start Page: | 1341 |
| End Page: | 1345 |
| Language: | English |
| DOI: | 10.1016/s0161-5890(02)00085-8 |
| PUBMED: | 12217405 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Presented at the 4th International Workshop on Signal Transduction in the Activation and Development of Mast Cells and Basophils; 2001 Nov 26-30; Bethesda, MD -- Export Date: 14 November 2014 -- Source: Scopus |
