Identification and characterization of a novel promoter of the mouse mu opioid receptor gene (Oprm) that generates eight splice variants Journal Article
| Author: | Pan, Y. X. |
| Article Title: | Identification and characterization of a novel promoter of the mouse mu opioid receptor gene (Oprm) that generates eight splice variants |
| Abstract: | Our previous studies isolated and characterized eight splicing variants containing exon 11 as the first coding exon. The location of exon 11 about 10 kb upstream from exon 1 dual promoters implied another promoter to drive expression of the exon 11 variants. I now identify the second promoter in the 5′-flanking region of exon 11. One major transcriptional start point was determined. Sequence analysis indicated that the 5′-flanking region of the exon 11 contained a putative TATA box, several CAAT boxes and a number of cis-acting elements. Functional analysis suggested that exon 11 promoter activity was most evident in neuronal-like cells. A basal core region containing the TATA box, a negative region and a positive region were identified. Electrophoretic mobility shift assays with the nuclear extracts from NIE-115 cells revealed several protein complexes that likely contained TATA box-associated factors, NF-1-like and cMyc-Max-like proteins, respectively. It also showed that a TATA-binding protein specifically bound to the TATA box fragment. Mutation analysis suggested that the TATA box in the basal core region played a fundamental role in the exon 11 promoter, whereas the NF-1 site acted as a positive element. © 2002 Elsevier Science B.V. All rights reserved. |
| Keywords: | controlled study; human cell; promoter region; sequence analysis; exon; mutation; exons; sequence deletion; nonhuman; animal cell; mouse; animals; mice; animal tissue; protein; protein binding; tumor cells, cultured; mutational analysis; nuclear proteins; dna; transcription regulation; molecular sequence data; gene identification; alternative splicing; base sequence; plasmids; dna flanking region; dna sequence; binding sites; gel mobility shift assay; electrophoretic mobility shift assay; protein variant; mu opiate receptor; receptors, opioid, mu; cis acting element; gene activity; transcription initiation site; sequence analysis, dna; cho cells; cricetinae; cell extract; tata box; nuclear factor; promoter regions (genetics); 5' flanking region; tata binding protein; tata-box binding protein; humans; human; priority journal; article; cmyc-max; nf-1 |
| Journal Title: | Gene |
| Volume: | 295 |
| Issue: | 1 |
| ISSN: | 0378-1119 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2002-07-24 |
| Start Page: | 97 |
| End Page: | 108 |
| Language: | English |
| DOI: | 10.1016/s0378-1119(02)00825-9 |
| PUBMED: | 12242016 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
