Identification and characterization of a novel promoter of a mouse mu opioid receptor gene (MOR-1) that directs alternative splicing and differential expression of eight new variants Meeting Abstract


Authors: Pan, Y.; Xu, J.; Rossi, G.; Xu, M.; Mahurter, L.; Bolan, E.; Pasternak, G. W.
Abstract Title: Identification and characterization of a novel promoter of a mouse mu opioid receptor gene (MOR-1) that directs alternative splicing and differential expression of eight new variants
Meeting Title: 30th Meeting of the Society for Neuroscience
Abstract: Using a modified 5'RACE approach, we isolated a novel exon (exon 11), which led to identify three additional exons (exons 12, 13 and 14) and eight novel splice variants (MOR-1G, -1H, -1I, -1J, -1K, -1L, -1M and MOR-1N) of the mouse MOR-1 gene. Exon 11 was mapped to upstream of exon 1 and the others between exons 11 and 2. Exon 11 is the first 5'-end exon for all eight new variants. They all underwent different splicing patterns yielding transcripts for a number of predicted proteins, including the original MOR-1. A single putative transcription start point was determined. Functional characterization of the 5'-flanking region of exon 11 by a SEAP reporter system in several cell lines revealed neuronal-specific promoter activities in the 5' flanking region, which contains a TATA box, two CAAT boxes and several binding sites including C/EBPbeta and AP-1 and cMyc/max. Northern blot analysis with an exon 11 probe shows a diffuse band from 4 to 7 kb. The differential regional distributions of the variant mRNAs, determined by RT-PCR, implied region-specific and/or cell-specific RNA processing. Antisense mapping using two probes targeting exon 11 had different actions spinally and supraspinally, blocking either morphine spinally or M6G supraspinally. Since exon 11 is present in all eight variants, it is not possible to assign a specific variant to these actions, but these findings do suggest that these new variants are pharmacologically relevant.
Keywords: meeting abstract; seap reporter system
Journal Title: Society for Neuroscience Abstracts
Volume: 26
Issue: Part 2
Meeting Dates: 2000 Nov 4-9
Meeting Location: New Orleans, LA
ISSN: 0190-5295
Publisher: Society for Neuroscience  
Date Published: 2000-01-01
Start Page: 2097
Language: English
ACCESSION: BCI:BCI200100135179
PROVIDER: biosis
Notes: Meeting Abstract: 783.2 -- 30th Annual Meeting of the Society of Neuroscience -- New Orleans, LA, USA -- November 04-09, 2000 -- Society for Neuroscience -- Source: Biosis