Transcriptional activating regions target a cyclin-dependent kinase Journal Article

   


Authors: Ansari, A. Z.; Koh, S. S.; Zaman, Z.; Bongards, C.; Lehming, N.; Young, R. A.; Ptashne, M.
Article Title: Transcriptional activating regions target a cyclin-dependent kinase
Abstract: Several yeast activators are phosphorylated by SRB10, a cyclin-dependent kinase associated with the transcriptional machinery. Sites of phosphorylation are found outside the activating region in each case, and the modification has different physiological consequences in different cases. We show here that certain acidic transcriptional activating regions contact SRB10 as assayed both in vivo and in vitro. The interaction evidently positions each activator, as it activates transcription, so that it gets phosphorylated by SRB10, and thus a common mechanism targets disparate substrates to the kinase.
Keywords: protein phosphorylation; protein targeting; transcription initiation; transcription, genetic; enzyme substrate; transcription factors; enzyme analysis; saccharomyces cerevisiae; protein biosynthesis; saccharomyces cerevisiae proteins; cyclin-dependent kinases; cyclin dependent kinase; enzyme binding; surface plasmon resonance; rna polymerase ii; trans-activation (genetics); priority journal; article
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 99
Issue: 23
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 2002-11-12
Start Page: 14706
End Page: 14709
Language: English
DOI: 10.1073/pnas.232573899
PUBMED: 12417740
PROVIDER: scopus
PMCID: PMC137483
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-0037069347&partnerID=40&md5=0e919aff973793d55dc03584e3090f78
Notes: Export Date: 14 November 2014 -- Source: Scopus
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MSK Authors
  1. Mark Ptashne
    61 Ptashne
  2. Aseem Z Ansari
    10 Ansari
  3. Zafar Zaman
    6 Zaman
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