Transcriptional activating regions target attached substrates to a cyclin-dependent kinase Journal Article
| Authors: | Ansari, A. Z.; Ogirala, A.; Ptashne, M. |
| Article Title: | Transcriptional activating regions target attached substrates to a cyclin-dependent kinase |
| Abstract: | The yeast cyclin-dependent kinase Srb10 phosphorylates various transcriptional activators as they activate transcription, and acidic transcriptional activating domains found on several activators directly bind Srb10. Here we show that the interaction between Srb10 (with its associated cyclin Srb11) and each of several different activating regions, in vitro, leads to the phosphorylation of peptide sequences attached to but outside of the activating regions themselves. In some cases, residues within the activating regions are also phosphorylated. The results define a mechanism by which a kinase is recruited to alternate substrates with diverse physiological consequences. |
| Keywords: | unclassified drug; protein domain; protein protein interaction; transcription initiation; in vitro study; enzyme substrate; phosphorylation; enzyme phosphorylation; amino acid sequence; molecular sequence data; recombinant fusion proteins; saccharomyces cerevisiae; substrate specificity; binding sites; trans-activators; yeast; saccharomyces cerevisiae proteins; cycline; cyclin-dependent kinases; cyclin dependent kinase; mutagenesis; enzyme mechanism; transcriptional regulation; proteolysis; kinase-substrate targeting; srb10/11; protein srb10; protein srb11 |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 102 |
| Issue: | 7 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2005-02-15 |
| Start Page: | 2346 |
| End Page: | 2349 |
| Language: | English |
| DOI: | 10.1073/pnas.0409671102 |
| PUBMED: | 15687503 |
| PROVIDER: | scopus |
| PMCID: | PMC549008 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 10" - "Export Date: 24 October 2012" - "CODEN: PNASA" - "Source: Scopus" |
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