An effect of DNA sequence on nucleosome occupancy and removal Journal Article
| Authors: | Wang, X.; Bryant, G. O.; Floer, M.; Spagna, D.; Ptashne, M. |
| Article Title: | An effect of DNA sequence on nucleosome occupancy and removal |
| Abstract: | A barrier phases nucleosomes at the yeast (Saccharomyces cerevisiae) GAL1-GAL10 genes. Here we separate nucleosome positioning from occupancy and show that the degree of occupancy of these phased sites is predictably determined by the underlying DNA sequences. As this occupancy is increased (by sequence alteration), nucleosome removal upon induction is decreased, as is mRNA production. These results explain why promoter sequences have evolved to form nucleosomes relatively inefficiently. © 2011 Nature America, Inc. All rights reserved. |
| Keywords: | controlled study; gene sequence; promoter region; mutation; nonhuman; messenger rna; saccharomyces cerevisiae; dna sequence; cell separation; fungal gene; nucleosome; gal1 gene; gal10 gene |
| Journal Title: | Nature Structural and Molecular Biology |
| Volume: | 18 |
| Issue: | 4 |
| ISSN: | 1545-9993 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2011-04-01 |
| Start Page: | 507 |
| End Page: | 509 |
| Language: | English |
| DOI: | 10.1038/nsmb.2017 |
| PROVIDER: | scopus |
| PMCID: | PMC3071895 |
| PUBMED: | 21378966 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 23 June 2011" - "CODEN: NSMBC" - "Source: Scopus" |
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