Regulation of a Mammalian gene bearing a CpG Island promoter and a distal enhancer Journal Article


Authors: Berrozpe, G.; Bryant, G.; Warpinski, K.; Ptashne, M.
Article Title: Regulation of a Mammalian gene bearing a CpG Island promoter and a distal enhancer
Abstract: A quantitative nucleosome occupancy assay revealed rules for nucleosome disposition in yeast and showed how disposition affects regulation of the GAL genes. Here, we show how those findings apply to the control of Kit, a mammalian gene. The Kit promoter lies in a CpG island, and its enhancer (active in mast cells) lies some 150 kb upstream. Nucleosomes form with especially high avidities at the Kit promoter, a reaction that, we surmise, ensures extremely low basal expression. In mast cells, transcriptional activators displace nucleosomes that areless tightly formed at the Kit enhancer. In turn, the active enhancer replaces a single Kit promoter nucleosome with the transcriptional machinery, thereby inducing transcription over 1,000-fold. As at the yeast GAL genes, the inhibitory effects of nucleosomes facilitate high factors of induction by mammalian activators working in the absence of specific repressors. © 2013 The Authors.
Journal Title: Cell Reports
Volume: 4
Issue: 3
ISSN: 2211-1247
Publisher: Cell Press  
Date Published: 2013-08-15
Start Page: 445
End Page: 453
Language: English
DOI: 10.1016/j.celrep.2013.07.001
PROVIDER: scopus
PUBMED: 23911283
DOI/URL:
Notes: --- - "Export Date: 4 September 2013" - "Source: Scopus"
Altmetric Score
MSK Authors
  1. Mark Ptashne
    61 Ptashne
  2. Gene Bryant
    14 Bryant