A RSC/nucleosome complex determines chromatin architecture and facilitates activator binding Journal Article
| Authors: | Floer, M.; Wang, X.; Prabhu, V.; Berrozpe, G.; Narayan, S.; Spagna, D.; Alvarez, D.; Kendall, J.; Krasnitz, A.; Stepansky, A.; Hicks, J.; Bryant, G. O.; Ptashne, M. |
| Article Title: | A RSC/nucleosome complex determines chromatin architecture and facilitates activator binding |
| Abstract: | How is chromatin architecture established and what role does it play in transcription? We show that the yeast regulatory locus UASg bears, in addition to binding sites for the activator Gal4, sites bound by the RSC complex. RSC positions a nucleosome, evidently partially unwound, in a structure that facilitates Gal4 binding to its sites. The complex comprises a barrier that imposes characteristic features of chromatin architecture. In the absence of RSC, ordinary nucleosomes encroach over the UASg and compete with Gal4 for binding. Taken with our previous work, the results show that both prior to and following induction, specific DNA-binding proteins are the predominant determinants of chromatin architecture at the GAL1/10 genes. RSC/nucleosome complexes are also found scattered around the yeast genome. Higher eukaryotic RSC lacks the specific DNA-binding determinants found on yeast RSC, and evidently Gal4 works in those organisms despite whatever obstacle broadly positioned nucleosomes present. © 2010 Elsevier Inc. |
| Keywords: | controlled study; unclassified drug; dna binding protein; promoter region; gene deletion; genetics; dna-binding proteins; nonhuman; genetic analysis; proteins; metabolism; protein binding; gene locus; transcription factor; hela cell; hela cells; mutational analysis; transcription factors; dna; saccharomyces cerevisiae; eukaryota; chromatin; chromatin immunoprecipitation; nucleotide sequence; transactivator protein; binding site; genome; temperature; real time polymerase chain reaction; trans-activators; gene induction; saccharomyces cerevisiae proteins; saccharomyces cerevisiae protein; gene insertion; dna binding; fungal protein; eukaryote; transcription factor gal4; chromatin structure; nucleosome; nucleosomes; galactokinase; protein uasg; gal1 protein, s cerevisiae; gal10 protein, s cerevisiae; rsc complex, s cerevisiae; regulatory sequence; regulatory elements, transcriptional |
| Journal Title: | Cell |
| Volume: | 141 |
| Issue: | 3 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 2010-04-01 |
| Start Page: | 407 |
| End Page: | 418 |
| Language: | English |
| DOI: | 10.1016/j.cell.2010.03.048 |
| PUBMED: | 20434983 |
| PROVIDER: | scopus |
| PMCID: | PMC3032599 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 8" - "Export Date: 20 April 2011" - "CODEN: CELLB" - "Source: Scopus" |
