Voriconazole inhibits fungal growth without impairing antigen presentation or T-cell activation Journal Article
| Authors: | Van Epps, H. L.; Feldmesser, M.; Pamer, E. G. |
| Article Title: | Voriconazole inhibits fungal growth without impairing antigen presentation or T-cell activation |
| Abstract: | Invasive aspergillosis (IA) is the most common life-threatening invasive mold infection worldwide. The principal therapy for IA is amphotericin B, despite its known toxicity and immunosuppressive side effects. Studies in animal models of IA suggest a role for T lymphocytes in the pathology of the disease, although the precise role for Aspergillus-specific T cells remains undefined. The isolation and characterization of T lymphocytes in animal models of IA are hampered by the rapid outgrowth of the fungus in cultures derived from infected organs. In the present study, we tested the abilities of the antifungal drugs caspofungin acetate and voriconazole to inhibit fungal growth in vitro as a means of maintaining cultures of T cells from Aspergillus-infected mice. We demonstrate that while both antifungal drugs are inhibitory, only voriconazole completely inhibited fungal growth, allowing long-term maintenance of T-cell cultures. In addition, voriconazole had no inhibitory effect on the activation and maturation of dendritic cells or the proliferation of T lymphocytes. Thus, voriconazole appears to be a promising agent for use in in vitro studies of Aspergillus-specific T lymphocytes in animal models of IA. |
| Keywords: | controlled study; drug efficacy; nonhuman; pathophysiology; lymphocyte proliferation; t-lymphocytes; animal cell; mouse; animals; mice; cell maturation; dendritic cell; culture medium; animal experiment; animal model; antifungal agent; in vitro study; drug effect; cell assay; pyrimidines; mice, inbred c57bl; cell specificity; mice, transgenic; disease model; antigen presentation; lymphocyte activation; dendritic cells; anti-bacterial agents; cell isolation; peptides; animal cell culture; cell stimulation; cell activation; caspofungin; voriconazole; antifungal agents; triazoles; t lymphocyte activation; aspergillus fumigatus; aspergillosis; peptides, cyclic; fungus growth; growth inhibition; amphotericin b; specific pathogen-free organisms; lymphocyte culture; priority journal; article |
| Journal Title: | Antimicrobial Agents and Chemotherapy |
| Volume: | 47 |
| Issue: | 6 |
| ISSN: | 0066-4804 |
| Publisher: | American Society for Microbiology |
| Date Published: | 2003-06-01 |
| Start Page: | 1818 |
| End Page: | 1823 |
| Language: | English |
| DOI: | 10.1128/aac.47.6.1818-1823.2003 |
| PUBMED: | 12760853 |
| PROVIDER: | scopus |
| PMCID: | PMC155840 |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work