A33scFV-cytosine deaminase: A recombinant protein construct for antibody-directed enzyme-prodrug therapy Journal Article
| Authors: | Deckert, P. M.; Renner, C.; Cohen, L. S.; Jungbluth, A.; Ritter, G.; Bertino, J. R.; Old, L. J.; Welt, S. |
| Article Title: | A33scFV-cytosine deaminase: A recombinant protein construct for antibody-directed enzyme-prodrug therapy |
| Abstract: | A recombinant fusion protein of colon carcinoma binding A33 single chain antibody with cytosine deaminase displayed specific antigen binding and enzyme activity in surface plasmon resonance and is catalytic activity assay. In vitro, it selectively increased the toxicity of 5-FC to A33 antigen-positive cells by 300-fold, demonstrating the potency of this ADEPT strategy. © 2003 Cancer Research UK. |
| Keywords: | controlled study; protein expression; unclassified drug; human cell; polymerase chain reaction; cytotoxicity; tumor cells, cultured; enzyme activity; immunoglobulin variable region; antigen; hybrid protein; antigens, neoplasm; recombinant fusion proteins; membrane glycoproteins; escherichia coli; recombinant protein; catalysis; colon carcinoma; antigen binding; surface plasmon resonance; antibody; single chain fragment variable antibody; prodrug; cytosine deaminase; flucytosine; immunoglobulin fragments; a33 antigen; a33 antibody; tumour targeting; antibody directed enzyme prodrug therapy; humans; human; priority journal; article; directed enzyme-producing therapy (adept); nucleoside deaminases |
| Journal Title: | British Journal of Cancer |
| Volume: | 88 |
| Issue: | 6 |
| ISSN: | 0007-0920 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2003-03-18 |
| Start Page: | 937 |
| End Page: | 939 |
| Language: | English |
| DOI: | 10.1038/sj.bjc.6600751 |
| PUBMED: | 12644833 |
| PROVIDER: | scopus |
| PMCID: | PMC2377093 |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work