Site-selective radiolabeling using mushroom tyrosinase and the strain-promoted oxidation-controlled 1,2-quinone cycloaddition Journal Article
| Authors: | Rodriguez, C.; Delaney, S.; Sebastiano, J.; Sarrett, S. M.; Cornejo, M. A.; Thau, S.; Hosny, M. M.; Zeglis, B. M. |
| Article Title: | Site-selective radiolabeling using mushroom tyrosinase and the strain-promoted oxidation-controlled 1,2-quinone cycloaddition |
| Abstract: | We report the in vitro characterization and in vivo evaluation of a novel Zr-89-labeled radioimmunoconjugate synthesized using a site-selective bioconjugation strategy based on the oxidation of tyrosinase residues exposed by the deglycosylation of the IgG and the subsequent strain-promoted oxidation-controlled 1,2-quinone cycloaddition between these amino acids and trans-cyclooctene-bearing cargoes. More specifically, we site-selectively modified a variant of the A33 antigen-targeting antibody huA33 with the chelator desferrioxamine (DFO), thereby producing an immunoconjugate (DFO-(SPOCQ)huA33) with equivalent antigen binding affinity to its parent immunoglobulin but attenuated affinity for the Fc gamma RI receptor. This construct was subsequently radiolabeled with [Zr-89]Zr4+ to create a radioimmunoconjugate - [Zr-89]Zr-DFO-(SPOCQ)huA33 - in high yield and specific activity that exhibited excellent in vivo behavior in two murine models of human colorectal carcinoma. |
| Keywords: | glycosylation; receptor; igg; binding |
| Journal Title: | RSC Advances |
| Volume: | 13 |
| Issue: | 26 |
| ISSN: | 2046-2069 |
| Publisher: | Royal Society of Chemistry |
| Date Published: | 2023-01-01 |
| Start Page: | 17705 |
| End Page: | 17709 |
| Language: | English |
| ACCESSION: | WOS:001004470000001 |
| DOI: | 10.1039/d3ra03486k |
| PROVIDER: | wos |
| PMCID: | PMC10258682 |
| PUBMED: | 37313000 |
| Notes: | Article -- Source: Wos |
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