The bioconjugation and radiosynthesis of (89)Zr-DFO-labeled antibodies Journal Article
| Authors: | Zeglis, B. M.; Lewis, J. S. |
| Article Title: | The bioconjugation and radiosynthesis of (89)Zr-DFO-labeled antibodies |
| Abstract: | The exceptional affinity, specificity, and selectivity of antibodies make them extraordinarily attractive vectors for tumor-targeted PET radiopharmaceuticals. Due to their multi-day biological half-life, antibodies must be labeled with positron-emitting radionuclides with relatively long physical decay half-lives. Traditionally, the positron-emitting isotopes124I (t1/2 = 4.18 d),86Y (t1/2 = 14.7 hr), and64Cu (t1/2 = 12.7 hr) have been used to label antibodies for PET imaging. More recently, however, the field has witnessed a dramatic increase in the use of the positron-emitting radiometal89Zr in antibody-based PET imaging agents.89Zr is a nearly ideal radioisotope for PET imaging with immunoconjugates, as it possesses a physical half-life (t1/2 = 78.4 hr) that is compatible with the in vivo pharmacokinetics of antibodies and emits a relatively low energy positron that produces high resolution images. Furthermore, antibodies can be straightforwardly labeled with89Zr using the siderophore-derived chelator desferrioxamine (DFO). In this protocol, the prostate-specific membrane antigen targeting antibody J591 will be used as a model system to illustrate (1) the bioconjugation of the bifunctional chelator DFO-isothiocyanate to an antibody, (2) the radiosynthesis and purification of a89Zr-DFO-mAb radioimmunoconjugate, and (3) in vivo PET imaging with an 89Zr-DFO-mAb radioimmunoconjugate in a murine model of cancer. © JoVE 2006-2015. All Rights Reserved. |
| Keywords: | positron emission tomography; chemistry; antibody; desferrioxamine; 89zr; immunoconjugates; bioconjugation; issue 96 |
| Journal Title: | Journal of Visualized Experiments |
| Issue: | 96 |
| ISSN: | 1940-087X |
| Publisher: | MYJoVE Corporation |
| Date Published: | 2015-02-12 |
| Start Page: | e52521 |
| Language: | English |
| DOI: | 10.3791/52521 |
| PROVIDER: | scopus |
| PUBMED: | 25741890 |
| PMCID: | PMC4354640 |
| DOI/URL: | |
| Notes: | Export Date: 2 April 2015 -- Source: Scopus |
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