Characterization of posttranslational modifications of human A33 antigen, a novel palmitoylated surface glycoprotein of human gastrointestinal epithelium Journal Article
| Authors: | Ritter, G.; Cohen, L. S.; Nice, E. C.; Catimel, B.; Burgess, A. W.; Moritz, R. L.; Ji, H.; Heath, J. K.; White, S. J.; Welt, S.; Old, L. J.; Simpson, R. J. |
| Article Title: | Characterization of posttranslational modifications of human A33 antigen, a novel palmitoylated surface glycoprotein of human gastrointestinal epithelium |
| Abstract: | Monoclonal antibody (mAb) A33 recognizes a differentiation antigen (A33) expressed in normal human gastrointestinal epithelium and in 95% of human colon cancers. Murine mAb A33 shows specific targeting of colon cancer in humans and a humanized A33 antibody is currently being evaluated in the clinic. The cDNA far the human A33 antigen has recently been cloned, and sequence comparison indicated that the A33 antigen is a novel human cell surface molecule of the immunoglobulin superfamily. Because mAb A33 recognizes a conformational epitope, only a partial characterization of the A33 antigen has been carried out to date. In this report we show that the A33 antigen is (I) N-glycosylated, containing approximately 8 K of N-linked carbohydrate and there is no evidence for O-glycosylation, sialylation or glycophosphatidylinositol, and (ii) S-acylated in vitro, incorporating [H-3] palmitic acid linked through a hydroxylamine-sensitive thioester bond. The S-palmitoylation may be involved in regulating the internalization process initiated by binding of mAb A33 to cell surface A33 antigen. (C) 1997 Academic Press. |
| Keywords: | proteins; receptor; identification; colon-cancer |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 236 |
| Issue: | 3 |
| ISSN: | 0006-291X |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 1997-07-30 |
| Start Page: | 682 |
| End Page: | 686 |
| Language: | English |
| ACCESSION: | WOS:A1997XQ53300030 |
| DOI: | 10.1006/bbrc.1997.6966 |
| PROVIDER: | wos |
| PUBMED: | 9245713 |
| Notes: | Article -- Source: Wos |
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