Structure of an Rrp6-RNA exosome complex bound to poly(A) RNA Journal Article

Authors: Wasmuth, E. V.; Januszyk, K.; Lima, C. D.
Article Title: Structure of an Rrp6-RNA exosome complex bound to poly(A) RNA
Abstract: The eukaryotic RNA exosome processes and degrades RNA by directing substrates to the distributive or processive 3' to 5' exoribonuclease activities of Rrp6 or Rrp44, respectively. The non-catalytic nine-subunit exosome core (Exo9) features a prominent central channel. Although RNA can pass through the channel to engage Rrp44, it is not clear how RNA is directed to Rrp6 or whether Rrp6 uses the central channel. Here we report a 3.3 Å crystal structure of a ten-subunit RNA exosome complex from Saccharomyces cerevisiae composed of the Exo9 core and Rrp6 bound to single-stranded poly(A) RNA. The Rrp6 catalytic domain rests on top of the Exo9 S1/KH ring above the central channel, the RNA 3' end is anchored in the Rrp6 active site, and the remaining RNA traverses the S1/KH ring in an opposite orientation to that observed in a structure of a Rrp44-containing exosome complex. Solution studies with human and yeast RNA exosome complexes suggest that the RNA path to Rrp6 is conserved and dependent on the integrity of the S1/KH ring. Although path selection to Rrp6 or Rrp44 is stochastic in vitro, the fate of a particular RNA may be determined in vivo by the manner in which cofactors present RNA to the RNA exosome. © 2014 Macmillan Publishers Limited. All rights reserved.
Keywords: unclassified drug; nonhuman; in vivo study; in vitro study; saccharomyces cerevisiae; crystal structure; rna structure; ribonuclease iii; enzyme active site; protein rna binding; rna degradation; exosome; single stranded rna; polyadenylated rna; human; priority journal; article; rrp 44 protein; rrp 6 protein
Journal Title: Nature
Volume: 511
Issue: 7510
ISSN: 0028-0836
Publisher: Nature Publishing Group  
Date Published: 2014-07-24
Start Page: 435
End Page: 439
Language: English
DOI: 10.1038/nature13406
PROVIDER: scopus
PUBMED: 25043052
PMCID: PMC4310248
Notes: Export Date: 2 September 2014 -- CODEN: NATUA -- Source: Scopus
Citation Impact
MSK Authors
  1. Christopher D Lima
    95 Lima
  2. Elizabeth Wasmuth
    10 Wasmuth