Generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos Journal Article

  


Authors: Artus, J.; Hadjantonakis, A. K.
Article Title: Generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos
Abstract: From the hybrid creatures of the Greek and Egyptian mythologies, the concept of the chimera has evolved and, in modern day biology, refers to an organism comprises of at least two populations of genetically distinct cells. Mouse chimeras have proven an invaluable tool for the generation of genetically modified strains. In addition, chimeras have been extensively used in developmental biology as a powerful tool to analyze the phenotype of specific mutations, to attribute function to gene products and to address the question of cell autonomy versus noncell autonomy of gene function. This chapter describes a simple and economical technique used to generate mouse chimeras by embryo aggregation. Multiple aggregation combinations are described each of which can be tailored to answer particular biological questions.
Keywords: mouse; animal; cytology; metabolism; animals; mice; embryonic stem cell; chimera; animal embryo; embryonic stem cells; embryo, mammalian; tetraploidy; diploidy
Journal Title: Methods in Molecular Biology
Volume: 693
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2011-01-01
Start Page: 37
End Page: 56
Language: English
PUBMED: 21080273
PROVIDER: scopus
DOI: 10.1007/978-1-60761-974-1_3
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-79952278792&partnerID=40&md5=564fb3488168425c06748c5dc09631b8
Notes: Chapter 3 in "Transgenic Mouse Methods and Protocols" (ISBN: 978-1-60761-973-4) --- Export Date: 23 June 2011 - Source: Scopus
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MSK Authors
  1. Anna-Katerina Hadjantonakis
    192 Hadjantonakis
  2. Jerome Francois Artus
    14 Artus
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