The amn gene product is required in extraembryonic tissues for the generation of middle primitive streak derivatives Journal Article

  

Authors:	Tomihara-Newberger, C.; Haub, O.; Lee, H. G.; Soares, V.; Manova, K.; Lacy, E.
Article Title:	The amn gene product is required in extraembryonic tissues for the generation of middle primitive streak derivatives
Abstract:	The primitive streak is the defining feature of the gastrulating mouse embryo. Currently, little is known in the mouse about the mechanisms that mediate the assembly of the primitive streak or about the signaling pathways that specify the different types of mesoderm and endoderm generated from the streak. To gain insight into primitive streak assembly and function, we have conducted a detailed phenotypic characterization of amnionless, a transgene- induced insertional mouse mutation that arrests embryonic development during gastrulation. Our histological and molecular analyses, when examined in the context of the mouse gastrula fate map, lead to the model that middle streak formation is specifically impaired in the amnionless mutant. Significantly, these observations argue that the formation of the middle streak is mediated by a pathway that is genetically separable from those that direct the specification of the distal and proximal streak regions. Intriguingly, our findings from wt ES cell mutually implies amnionless(-/-) blastocyst chimeras indicate that this proposed separate pathway for middle streak formation is dependent on amnionless gene functions in the visceral endoderm.
Keywords:	gene mutation; mutation; nonhuman; mouse; mammalia; animals; mice; animal tissue; gene expression; embryo; membrane proteins; genotype; gene product; cell differentiation; animalia; mice, transgenic; chimera; gene expression regulation, developmental; visceral endoderm; endoderm; blastocyst; gastrulation; primitive streak; gastrula; priority journal; article; embryonic and fetal development; mouse gastrulation
Journal Title:	Developmental Biology
Volume:	204
Issue:	1
ISSN:	0012-1606
Publisher:	Elsevier Inc.
Date Published:	1998-12-01
Start Page:	34
End Page:	54
Language:	English
DOI:	10.1006/dbio.1998.9034
PUBMED:	9851841
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032387124&doi=10.1006%2fdbio.1998.9034&partnerID=40&md5=e4572b07c6c47094172f72cce44283e2
Notes:	Article -- Export Date: 12 December 2016 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/102296