Production of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos Journal Article
| Authors: | Eakin, G. S.; Hadjantonakis, A. K. |
| Article Title: | Production of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos |
| Abstract: | The production of mouse chimeras is a common step in the establishment of genetically modified animal strains. Chimeras also provide a powerful experimental tool for following cell behavior during both prenatal and postnatal development. This protocol outlines a simple and economical technique for the production of large numbers of mouse chimeras using traditional diploid morula↔diploid embryonic stem (ES) cell aggregations. Additional steps are included to describe the procedures necessary to produce specialized tetraploid chimeras using tetraploid morula↔diploid ES cell aggregations. This increasingly popular form of chimera produces embryos of nearly complete ES cell derivation that can be used to speed transgenic production or ask developmental questions. Using this protocol, mouse chimeras can be generated and transferred to pseudopregnant surrogate mothers in a 5-d period. |
| Keywords: | genetics; mouse; animal; cytology; animals; mice; embryonic stem cell; animal model; embryo development; physiology; chimera; models, animal; prenatal development; embryonic stem cells; embryonic development; morula |
| Journal Title: | Nature Protocols |
| Volume: | 1 |
| Issue: | 3 |
| ISSN: | 1754-2189 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2006-01-01 |
| Start Page: | 1145 |
| End Page: | 1153 |
| Language: | English |
| DOI: | 10.1038/nprot.2006.173 |
| PUBMED: | 17406396 |
| PROVIDER: | scopus |
| PMCID: | PMC2883166 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 19" - "Export Date: 4 June 2012" - "Source: Scopus" |
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