Dicer-independent, Ago2-mediated microRNA biogenesis in vertebrates Journal Article
| Authors: | Yang, J. S.; Lai, E. C. |
| Article Title: | Dicer-independent, Ago2-mediated microRNA biogenesis in vertebrates |
| Abstract: | A canonical biogenesis pathway involving sequential cleavage by the Drosha and Dicer RNAse III enzymes governs the maturation of most animal microRNAs. However, there exist a variety of alternative miRNA biogenesis pathways, most of which bypass Drosha processing. Recently, three groups described for the first time a vertebrate microRNA pathway that bypasses Dicer cleavage. This mechanism was characterized with respect to the highly conserved vertebrate gene mir-451, for which Drosha processing yields a short (42 nucleotide) hairpin that is directly loaded into Ago2, the sole vertebrate "Slicer" Argonaute. Ago2-mediated cleavage of this hairpin yields a 30 nucleotide intermediate, whose 3′ end is resected to generate the dominantly cloned ∼23 nucleotide mature miR-451. Knowledge of this pathway provides an unprecedented tool with which to express microRNAs and small interfering RNAs in Dicer mutant cells. More generally, the mir-451 backbone constitutes a new platform for gene silencing that complements existing shRNA technology. © 2010 Landes Bioscience. |
| Keywords: | unclassified drug; review; nonhuman; microrna; gene expression; erythropoiesis; small interfering rna; gene locus; animalia; vertebrata; molecular cloning; gene silencing; vertebrate; short hairpin rna; biogenesis; gene structure; dicer; argonaute 2 protein; ribonuclease; mir-451; slicer; microrna 451; ago2; dicer-independent; protein drosha; dna hairpin |
| Journal Title: | Cell Cycle |
| Volume: | 9 |
| Issue: | 22 |
| ISSN: | 1538-4101 |
| Publisher: | Taylor & Francis Inc. |
| Date Published: | 2010-11-15 |
| Start Page: | 4455 |
| End Page: | 4460 |
| Language: | English |
| DOI: | 10.4161/cc.9.22.13958 |
| PROVIDER: | scopus |
| PMCID: | PMC3048044 |
| PUBMED: | 21088485 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 2" - "Export Date: 20 April 2011" - "Source: Scopus" |
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