The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila Journal Article

Authors: Okamura, K.; Hagen, J. W.; Duan, H.; Tyler, D. M.; Lai, E. C.
Article Title: The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila
Abstract: The canonical microRNA (miRNA) pathway converts primary hairpin precursor transcripts into ∼22 nucleotide regulatory RNAs via consecutive cleavages by two RNase III enzymes, Drosha and Dicer. In this study, we characterize Drosophila small RNAs that derive from short intronic hairpins termed "mirtrons." Their nuclear biogenesis appears to bypass Drosha cleavage, which is essential for miRNA biogenesis. Instead, mirtron hairpins are defined by the action of the splicing machinery and lariat-debranching enzyme, which yield pre-miRNA-like hairpins. The mirtron pathway merges with the canonical miRNA pathway during hairpin export by Exportin-5, and both types of hairpins are subsequently processed by Dicer-1/loqs. This generates small RNAs that can repress perfectly matched and seed-matched targets, and we provide evidence that they function, at least in part, via the RNA-induced silencing complex effector Ago1. These findings reveal that mirtrons are an alternate source of miRNA-type regulatory RNAs. © 2007 Elsevier Inc. All rights reserved.
Keywords: signal transduction; nonhuman; animals; microrna; signaling; intron; introns; drosophila; structure-activity relationship; evolution, molecular; gene expression regulation; molecular sequence data; recombinant fusion proteins; base sequence; models, genetic; drosophila melanogaster; gene silencing; nucleic acid conformation; micrornas; drosophila proteins; humdisease; rna splicing; rna precursors; biogenesis; dicer; ribonuclease iii; animals, genetically modified; exportin 5
Journal Title: Cell
Volume: 130
Issue: 1
ISSN: 0092-8674
Publisher: Cell Press  
Date Published: 2007-07-13
Start Page: 89
End Page: 100
Language: English
DOI: 10.1016/j.cell.2007.06.028
PUBMED: 17599402
PROVIDER: scopus
PMCID: PMC2729315
Notes: --- - "Cited By (since 1996): 206" - "Export Date: 17 November 2011" - "CODEN: CELLB" - "Source: Scopus"
Citation Impact
MSK Authors
  1. David M Tyler
    6 Tyler
  2. Eric C Lai
    135 Lai
  3. Hong Duan
    11 Duan
  4. Katsutomo Okamura
    26 Okamura
  5. Joshua W Hagen
    7 Hagen