MicroRNA biogenesis via splicing and exosome-mediated trimming in Drosophila Journal Article

Authors: Flynt, A. S.; Greimann, J. C.; Chung, W. J.; Lima, C. D.; Lai, E. C.
Article Title: MicroRNA biogenesis via splicing and exosome-mediated trimming in Drosophila
Abstract: microRNAs (miRNAs) are approximately 22 nucleotide regulatory RNAs derived from hairpins generated either by Drosha cleavage (canonical substrates) or by splicing and debranching of short introns (mirtrons). The 5' end of the highly conserved Drosophila mirtron-like locus mir-1017 is coincident with the splice donor, but a substantial "tail" separates its hairpin from the 3'splice acceptor. Genetic and biochemical studies define a biogenesis pathway involving splicing, lariat debranching, and RNA exosome-mediated "trimming," followed by conventional dicing and loading into AGO1 to yield a miRNA that can repress seed-matched targets. Analysis of cloned small RNAs yielded six additional candidate 3' tailed mirtrons in D. melanogaster. Altogether, these data reveal an unexpected role for the exosome in the biogenesis of miRNAs from hybrid mirtron substrates. Copyright (c) 2010 Elsevier Inc. All rights reserved.
Keywords: genetics; molecular genetics; animal; metabolism; animals; microrna; intron; introns; physiology; molecular sequence data; nucleotide sequence; base sequence; genome; drosophila melanogaster; micrornas; drosophila proteins; rna splicing; drosophila protein; eukaryotic initiation factors; genome, insect; argonaute1 protein, drosophila; initiation factor; exosome; exosomes
Journal Title: Molecular Cell
Volume: 38
Issue: 6
ISSN: 1097-2765
Publisher: Cell Press  
Date Published: 2010-06-25
Start Page: 900
End Page: 907
Language: English
PUBMED: 20620959
PROVIDER: scopus
PMCID: PMC2904328
DOI: 10.1016/j.molcel.2010.06.014
Notes: --- - "Cited By (since 1996): 6" - "Export Date: 20 April 2011" - "Source: Scopus"
Citation Impact
MSK Authors
  1. Eric C Lai
    152 Lai
  2. Alex S Flynt
    12 Flynt
  3. Christopher D Lima
    100 Lima
  4. Weijen Chung
    9 Chung