MicroRNA biogenesis via splicing and exosome-mediated trimming in Drosophila Journal Article
| Authors: | Flynt, A. S.; Greimann, J. C.; Chung, W. J.; Lima, C. D.; Lai, E. C. |
| Article Title: | MicroRNA biogenesis via splicing and exosome-mediated trimming in Drosophila |
| Abstract: | microRNAs (miRNAs) are approximately 22 nucleotide regulatory RNAs derived from hairpins generated either by Drosha cleavage (canonical substrates) or by splicing and debranching of short introns (mirtrons). The 5' end of the highly conserved Drosophila mirtron-like locus mir-1017 is coincident with the splice donor, but a substantial "tail" separates its hairpin from the 3'splice acceptor. Genetic and biochemical studies define a biogenesis pathway involving splicing, lariat debranching, and RNA exosome-mediated "trimming," followed by conventional dicing and loading into AGO1 to yield a miRNA that can repress seed-matched targets. Analysis of cloned small RNAs yielded six additional candidate 3' tailed mirtrons in D. melanogaster. Altogether, these data reveal an unexpected role for the exosome in the biogenesis of miRNAs from hybrid mirtron substrates. Copyright (c) 2010 Elsevier Inc. All rights reserved. |
| Keywords: | genetics; molecular genetics; animal; metabolism; animals; microrna; intron; introns; physiology; molecular sequence data; nucleotide sequence; base sequence; genome; drosophila melanogaster; micrornas; drosophila proteins; rna splicing; drosophila protein; eukaryotic initiation factors; genome, insect; argonaute1 protein, drosophila; initiation factor; exosome; exosomes |
| Journal Title: | Molecular Cell |
| Volume: | 38 |
| Issue: | 6 |
| ISSN: | 1097-2765 |
| Publisher: | Cell Press |
| Date Published: | 2010-06-25 |
| Start Page: | 900 |
| End Page: | 907 |
| Language: | English |
| PUBMED: | 20620959 |
| PROVIDER: | scopus |
| PMCID: | PMC2904328 |
| DOI: | 10.1016/j.molcel.2010.06.014 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 6" - "Export Date: 20 April 2011" - "Source: Scopus" |
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