The utility of immunohistochemical detection of DNA mismatch repair gene proteins Journal Article
| Authors: | Shia, J.; Ellis, N. A.; Klimstra, D. S. |
| Article Title: | The utility of immunohistochemical detection of DNA mismatch repair gene proteins |
| Abstract: | Since the development of monoclonal antibodies against the MSH2 protein by Leach et al. in 1996, a series of investigations has been undertaken to determine the utility of immunohistochemical detection of DNA mismatch repair (MMR) gene proteins in the identification of hereditary or sporadic colorectal tumors with microsatellite instability. These studies, however, have been performed with different aims and on different patient populations. Interpretation of these immunohistochemical data relies on a thorough understanding of the biological and technical factors that affect the detection of MMR proteins. In this review, we analyze the data from the published research studies, pointing out the various factors affecting immunohistochemical detection of MMR proteins and projecting the utility of immunohistochemistry in different clinical settings. © Springer-Verlag 2004. |
| Keywords: | immunohistochemistry; protein expression; gene mutation; promoter region; missense mutation; dna-binding proteins; proto-oncogene proteins; review; cancer risk; nonhuman; sensitivity and specificity; sensitivity analysis; phenotype; dna repair; neoplasm proteins; genetic variability; risk factor; nuclear proteins; cancer genetics; correlation analysis; statistical significance; quantitative analysis; mismatch repair; microsatellite instability; gene identification; carrier proteins; staining; gene control; high risk population; muts homolog 2 protein; genetic linkage; heritability; base pair mismatch; humans; human; priority journal; dna mismatch repair gene proteins |
| Journal Title: | Virchows Archiv |
| Volume: | 445 |
| Issue: | 5 |
| ISSN: | 0945-6317 |
| Publisher: | Springer |
| Date Published: | 2004-11-01 |
| Start Page: | 431 |
| End Page: | 441 |
| Language: | English |
| DOI: | 10.1007/s00428-004-1090-5 |
| PROVIDER: | scopus |
| PUBMED: | 15455227 |
| DOI/URL: | |
| Notes: | Virchows Arch. -- Cited By (since 1996):41 -- Export Date: 16 June 2014 -- CODEN: VARCE -- Source: Scopus |
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