DNA mismatch repair deficiency in ampullary carcinoma: A morphologic and immunohistochemical study of 54 cases Journal Article
| Authors: | Agaram, N. P.; Shia, J.; Tang, L. H.; Klimstra, D. S. |
| Article Title: | DNA mismatch repair deficiency in ampullary carcinoma: A morphologic and immunohistochemical study of 54 cases |
| Abstract: | The significance of DNA mismatch repair (MMR) deficiency or microsatellite instability (MSI) in ampullary carcinomas remains to be defined. This study evaluated the MMR status in 54 consecutive ampullary adenocarcinomas by immunohistochemical and morphologic studies. All tumors were moderately (n = 49) or poorly (n = 5) differentiated, with 7 mucinous and 1 signet-ring cell type. Tumor-infiltrating lymphocytes (TILs) were noted in 36 tumors. Loss of MMR protein by immunohistochemical analysis was identified in 3 (6%), 2 lost MSH6, and 1 lost MLH1/PMS2. One MSH6- case had 3 metachronous colorectal cancers. Five TILs per 10 high-power fields predicted immunohistochemical abnormality in 2 of 3 tumors with a specificity of 80% (41/51); however, none of the 5 tumors that had the highest TIL counts (20-62/10 high-power fields) showed abnormal immunohistochemical results. Thus, MMR deficiency occurs in ampullary carcinoma but appears less frequent than in colorectal carcinoma (CRC). Typical MSI-high histologic features of CRC, such as increased TIL counts, seem to have similar yet subtly different implications in ampullary carcinoma. © American Society for Clinical Pathology. |
| Keywords: | immunohistochemistry; adult; human tissue; aged; aged, 80 and over; middle aged; dna binding protein; major clinical study; genetics; dna-binding proteins; sensitivity and specificity; adenocarcinoma; tumor associated leukocyte; metabolism; nuclear protein; tumor markers, biological; pathology; tumor marker; nuclear proteins; colorectal carcinoma; cancer genetics; adenoma; mismatch repair; microsatellite instability; dna mismatch repair; adaptor proteins, signal transducing; cell nucleus; signal transducing adaptor protein; mlh1 protein, human; lynch syndrome; protein msh2; g t mismatch binding protein; g-t mismatch-binding protein; msh2 protein, human; muts homolog 2 protein; hereditary nonpolyposis colorectal cancer; vater papilla carcinoma; vater papilla; microsatellite instability (high frequency) histology; bile duct tumor; ampulla of vater; common bile duct neoplasms |
| Journal Title: | American Journal of Clinical Pathology |
| Volume: | 133 |
| Issue: | 5 |
| ISSN: | 0002-9173 |
| Publisher: | Oxford University Press |
| Date Published: | 2010-05-01 |
| Start Page: | 772 |
| End Page: | 780 |
| Language: | English |
| DOI: | 10.1309/ajcpgdde8plldrcc |
| PUBMED: | 20395525 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Export Date: 20 April 2011" - "CODEN: AJCPA" - "Source: Scopus" |
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