Genetic variants in SOD2, MPO, and NQO1, and risk of ovarian cancer Journal Article
| Authors: | Olson, S. H.; Carlson, M. D. A.; Ostrer, H.; Harlap, S.; Stone, A.; Winters, M.; Ambrosone, C. B. |
| Article Title: | Genetic variants in SOD2, MPO, and NQO1, and risk of ovarian cancer |
| Abstract: | Objective. One way in which parity and use of oral contraceptives may protect against ovarian cancer is by preventing inflammation and oxidative stress associated with ovulation. Since the genes superoxide dismutase (SOD2), myeloperoxidase (MPO), and NAD(P)H:quinone oxidoreductase 1 (NQO1) are involved in inflammation and oxidative stress, we investigated whether variants of these genes are associated with risk of ovarian cancer. Methods. In a hospital-based case-control study, we compared 125 cases and 193 controls with respect to prevalence of (1) the T→C (val→ala) substitution at the -9 position in the signal sequence of SOD2; (2) the G→A substitution at the -463 position in the promoter region of MPO; and (3) the C→T (pro→ser) change in exon 6 of NQO1. Genotyping was done using PCR and gel electrophoresis for MPO and NQO1 and using MALDI-TOF mass spectrometry for SOD2. Results. For SOD2, women with the TC (val/ala) or CC (ala/ala) genotypes were at increased risk [odds ratio (OR) 2.1, 95% confidence interval (CI) 1.1-4.0]. Results for MPO and NQO1 were in the hypothesized directions but were not statistically significant. For MPO, there was a small inverse association among women with GA or AA genotypes (OR = 0.72, 95% CI 0.43-1.2). For NQO1, the TT (ser/ser) genotype was associated with somewhat increased risk (OR = 2.3, 95% CI 0.69-7.6). Conclusions. While these results need to be confirmed in other studies, they point to a possible role for genes involved in oxidative stress in the development of ovarian cancer. © 2004 Elsevier Inc. All rights reserved. |
| Keywords: | adult; controlled study; middle aged; major clinical study; promoter region; exon; case-control studies; cancer risk; polymerase chain reaction; ovarian neoplasms; peroxidase; ovary cancer; amino acid substitution; prevalence; myeloperoxidase; inflammation; genetic variability; genotype; alleles; risk factors; substitution reaction; risk assessment; confidence interval; statistical significance; signal peptide; oxidative stress; ovarian carcinoma; genetic risk; matrix assisted laser desorption ionization time of flight mass spectrometry; superoxide dismutase; polymorphism, genetic; genetic polymorphisms; gel electrophoresis; manganese superoxide dismutase; reduced nicotinamide adenine dinucleotide (phosphate) dehydrogenase (quinone); nad(p)h dehydrogenase (quinone); variation (genetics); humans; human; female; priority journal; article; nad(p)h:oxidoreductase 1 |
| Journal Title: | Gynecologic Oncology |
| Volume: | 93 |
| Issue: | 3 |
| ISSN: | 0090-8258 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2004-06-01 |
| Start Page: | 615 |
| End Page: | 620 |
| Language: | English |
| DOI: | 10.1016/j.ygyno.2004.03.027 |
| PROVIDER: | scopus |
| PUBMED: | 15196853 |
| DOI/URL: | |
| Notes: | Gynecol. Oncol. -- Cited By (since 1996):55 -- Export Date: 16 June 2014 -- CODEN: GYNOA -- Source: Scopus |
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