Plasmid-based short-hairpin RNA interference in the chicken embryo Journal Article


Authors: Chesnutt, C.; Niswander, L.
Article Title: Plasmid-based short-hairpin RNA interference in the chicken embryo
Abstract: The chicken neural tube serves as an ideal model for new techniques to alter gene expression. We show that short-hairpin RNA delivered by a plasmid vector can effectively knock down expression of both exogenous and endogenous genes in the chicken neural tube. We also assay the effects of short-hairpin RNA on the interferon response and find no difference between electroporation of a control plasmid and a short-hairpin RNA plasmid. © 2004 Wiley-Liss, Inc.
Keywords: unclassified drug; interferon; nonhuman; animals; mice; animal tissue; gene expression; embryo; nerve tissue proteins; small interfering rna; rna, small interfering; rna interference; homeodomain proteins; rna; gene expression regulation, developmental; green fluorescent proteins; electroporation; plasmids; short hairpin rna; model; plasmid vector; neural tube; knockout gene; chick embryo; nervous system; sirna; chicken; shrna; gene delivery system; rna polymerase iii; interferons; priority journal; article; gallus gallus
Journal Title: Genesis
Volume: 39
Issue: 2
ISSN: 1526-954X
Publisher: Wiley Blackwell  
Date Published: 2004-05-01
Start Page: 73
End Page: 78
Language: English
DOI: 10.1002/gene.20028
PROVIDER: scopus
PUBMED: 15170691
DOI/URL:
Notes: Genesis -- Cited By (since 1996):30 -- Export Date: 16 June 2014 -- CODEN: GNESF -- Source: Scopus
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