Plasmid-based short-hairpin RNA interference in the chicken embryo Journal Article
| Authors: | Chesnutt, C.; Niswander, L. |
| Article Title: | Plasmid-based short-hairpin RNA interference in the chicken embryo |
| Abstract: | The chicken neural tube serves as an ideal model for new techniques to alter gene expression. We show that short-hairpin RNA delivered by a plasmid vector can effectively knock down expression of both exogenous and endogenous genes in the chicken neural tube. We also assay the effects of short-hairpin RNA on the interferon response and find no difference between electroporation of a control plasmid and a short-hairpin RNA plasmid. © 2004 Wiley-Liss, Inc. |
| Keywords: | unclassified drug; interferon; nonhuman; animals; mice; animal tissue; gene expression; embryo; nerve tissue proteins; small interfering rna; rna, small interfering; rna interference; homeodomain proteins; rna; gene expression regulation, developmental; green fluorescent proteins; electroporation; plasmids; short hairpin rna; model; plasmid vector; neural tube; knockout gene; chick embryo; nervous system; sirna; chicken; shrna; gene delivery system; rna polymerase iii; interferons; priority journal; article; gallus gallus |
| Journal Title: | Genesis |
| Volume: | 39 |
| Issue: | 2 |
| ISSN: | 1526-954X |
| Publisher: | Wiley Blackwell |
| Date Published: | 2004-05-01 |
| Start Page: | 73 |
| End Page: | 78 |
| Language: | English |
| DOI: | 10.1002/gene.20028 |
| PROVIDER: | scopus |
| PUBMED: | 15170691 |
| DOI/URL: | |
| Notes: | Genesis -- Cited By (since 1996):30 -- Export Date: 16 June 2014 -- CODEN: GNESF -- Source: Scopus |
