Impact of RNA-guided technologies for target identification and deconvolution Journal Article
| Authors: | Fennell, M.; Xiang, Q.; Hwang, A.; Chen, C.; Huang, C. H.; Chen, C. C.; Pelossof, R.; Garippa, R. J. |
| Article Title: | Impact of RNA-guided technologies for target identification and deconvolution |
| Abstract: | For well over a decade, RNA interference (RNAi) has provided a powerful tool for investigators to query specific gene targets in an easily modulated loss-of-function setting, both in vitro and in vivo. Hundreds of publications have demonstrated the utility of RNAi in arrayed and pooled-based formats, in a wide variety of cell-based systems, including clonal, stem, transformed, and primary cells. Over the years, there have been significant improvements in the design of target-specific small-interfering RNA (siRNA) and short-hairpin RNA (shRNA), expression vectors, methods for mitigating off-target effects, and accurately interpreting screening results. Recent developments in RNAi technology include the Sensor assay, high-efficiency miR-E shRNAs, improved shRNA virus production with Pasha (DRGC8) knockdown, and assessment of RNAi off-target effects by using the C9-11 method. An exciting addition to the arsenal of RNA-mediated gene modulation is the clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas) system for genomic editing, allowing for gene functional knockout rather than knockdown. |
| Keywords: | review; nonhuman; microrna; small interfering rna; rna interference; molecular library; genetic transfection; screening; genome; gene silencing; genetic screening; short hairpin rna; genomic dna; functional genomics; virus vector; expression vector; rnai; gene technology; protein denaturation; viral vectors; rna editing; hcs; deconvolution; human; ote; crispr/cas9; pooled shrna; crispr cas system |
| Journal Title: | Journal of Biomolecular Screening |
| Volume: | 19 |
| Issue: | 10 |
| ISSN: | 1087-0571 |
| Publisher: | Sage Publications |
| Date Published: | 2014-12-01 |
| Start Page: | 1327 |
| End Page: | 1337 |
| Language: | English |
| DOI: | 10.1177/1087057114548414 |
| PROVIDER: | scopus |
| PUBMED: | 25163683 |
| DOI/URL: | |
| Notes: | Export Date: 2 January 2015 -- Source: Scopus |
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