Caspase-3 has a nonapoptotic function in erythroid maturation Journal Article
| Authors: | Carlile, G. W.; Smith, D. H.; Wiedmann, M. |
| Article Title: | Caspase-3 has a nonapoptotic function in erythroid maturation |
| Abstract: | Caspase-3 plays a central role in apoptosis. It is also activated in normal erythropoiesis, with its activity peaking early during development (erythroid colony-forming unit [CFU-E] stage). In the present study, we have reduced the expression and subsequent enzymatic activity of caspase-3 by transfection of small interfering RNA (siRNA) directed to caspase-3 in a differentiating human erythroid culture system. We find that siRNA treatment yields a 50% reduction in cells that undergo enucleation with no change in the fraction of cells that undergo apoptosis, measured throughout the culture. Furthermore, a substantial fraction of treated cells are unable to complete the transition from pronormoblasts to basophilic normoblasts. These results demonstrate that caspase-3 is required for efficient erythropoiesis in this model system. © 2004 by The American Society of Hematology. |
| Keywords: | controlled study; protein expression; human cell; cells, cultured; apoptosis; cell maturation; erythroblast; erythropoiesis; small interfering rna; rna, small interfering; rna interference; cell differentiation; caspase 3; enzyme activation; enzyme activity; caspases; cell assay; human cell culture; genetic transfection; membrane glycoproteins; erythroid cell; erythroblasts; antigens, cd; antigens, cd24; basophil; humans; human; priority journal; article; colony forming unit e |
| Journal Title: | Blood |
| Volume: | 103 |
| Issue: | 11 |
| ISSN: | 0006-4971 |
| Publisher: | American Society of Hematology |
| Date Published: | 2004-06-01 |
| Start Page: | 4310 |
| End Page: | 4316 |
| Language: | English |
| DOI: | 10.1182/blood-2003-09-3362 |
| PROVIDER: | scopus |
| PUBMED: | 14976035 |
| DOI/URL: | |
| Notes: | Blood -- Cited By (since 1996):88 -- Export Date: 16 June 2014 -- CODEN: BLOOA -- Source: Scopus |
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