6-shogaol protects against oxidized LDL-induced endothelial injruries by inhibiting oxidized LDL-evoked LOX-1 signaling Journal Article


Authors: Wang, Y. K.; Hong, Y. J.; Yao, Y. H.; Huang, X. M.; Liu, X. B.; Zhang, C. Y.; Zhang, L.; Xu, X. L.
Article Title: 6-shogaol protects against oxidized LDL-induced endothelial injruries by inhibiting oxidized LDL-evoked LOX-1 signaling
Abstract: Endothelial dysfunction and oxLDL are believed to be early and critical events in atherogenesis. 6-Shogaol is the major bioactive compound present in Zingiber officinale and possesses the anti-atherosclerotic effect. However, the mechanisms remain poorly understood. The goal of this study was to investigate the effects of 6-shogaol on oxLDL-induced Human umbilical vein endothelial cells (HUVECs) injuries and its possible molecular mechanisms. Hence, we studied the effects of 6-shogaol on cell apoptosis, cellular reactive oxygen species (ROS), NF-B activation, Bcl-2 expression, and caspase -3, -8, -9 activities. In addition, E-selectin, MCP-1, and ICAM-1 were determined by ELISA. Our study show that oxLDL increased LOX-1 expression, ROS levels, NF-B, caspases-9 and -3 activation and decreased Bcl-2 expression in HUVECs. These alterations were attenuated by 6-shogaol. Cotreatment with 6-shogaol and siRNA of LOX-1 synergistically reduced oxLDL-induced caspases -9, -3 activities and cell apoptosis. Overexpression of LOX-1 attenuated the protection by 6-shogaol and suppressed the effects of 6-shogaol on oxLDL-induced oxidative stress. In addition, oxLDL enhanced the activation of NF-B and expression of adhesion molecules. Pretreatment with 6-shogaol, however, exerted significant cytoprotective effects in all events. Our data indicate that 6-shogaol might be a potential natural antiapoptotic agent for the treatment of atherosclerosis. © 2013 Yun kai Wang et al.
Journal Title: Evidence-Based Complementary and Alternative Medicine
Volume: 2013
ISSN: 1741-427X
Publisher: Hindawi Publishing Corporation  
Date Published: 2013-01-01
Start Page: 503521
Language: English
DOI: 10.1155/2013/503521
PROVIDER: scopus
PMCID: PMC3590502
PUBMED: 23533490
DOI/URL:
Notes: --- - "Export Date: 1 April 2013" - "Source: Scopus"
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  1. Xiaoliang Xu
    15 Xu