Revisiting old drugs as novel agents for retinoblastoma: In vitro and in vivo antitumor activity of cardenolides Journal Article
| Authors: | Antczak, C.; Kloepping, C.; Radu, C.; Genski, T.; Müller-Kuhrt, L.; Siems, K.; de Stanchina, E.; Abramson, D. H.; Djaballah, H. |
| Article Title: | Revisiting old drugs as novel agents for retinoblastoma: In vitro and in vivo antitumor activity of cardenolides |
| Abstract: | PURPOSE. Intra-arterial delivery of chemotherapeutic agents offers a new and exciting opportunity for the treatment of advanced intraocular retinoblastoma. It allows local delivery of relatively high doses of chemotherapy agents while bypassing general blood circulation. For this reason, this study was undertaken to revisit some of the FDA-approved drugs for the treatment of retinoblastoma. METHODS. High-throughput screening (HTS) of 2640 approved drugs and bioactive compounds resulted in the identification of cytotoxic agents with potent activity toward both the Y79 and RB355 human retinoblastoma cell lines. Subsequent profiling of the drug candidates was performed in a panel of ocular cancer cell lines. Induction of apoptosis in Y79 cells was assessed by immunofluorescence detection of activated caspase-3. Therapeutic effect was evaluated in a xenograft model of retinoblastoma. RESULTS. Several FDA-approved drugs were identified that showed potent cytotoxic activity toward retinoblastoma cell lines in vitro. Among them were several cardiac glycosides, a class of cardenolides historically associated with the prevention and treatment of congestive heart failure. Caspase-3 activation studies provided an insight into the mechanism of action of cardenolides in retinoblastoma cells. When tested in a xenograft model of retinoblastoma, the cardenolide ouabain induced complete tumor regression in the treated mice. CONCLUSIONS. Cardenolides were identified as a new class of antitumor agents for the treatment of retinoblastoma. Members of this class of cardiotonic drugs could be repositioned for retinoblastoma if administered locally via direct intra-arterial infusion. © Association for Research in Vision and Ophthalmology. |
| Keywords: | controlled study; unclassified drug; human cell; cisplatin; dose response; drug dose comparison; nonhuman; antineoplastic agents; drug approval; antineoplastic agent; mouse; animal; metabolism; mouse mutant; animals; mice; carboplatin; apoptosis; tumor volume; etoposide; animal experiment; animal model; vincristine; in vivo study; calcitriol; cardenolide derivative; cardiac glycoside; caspase 3; digoxigenin; digoxin; gambogic acid; neriifollin; nigericin; nutlin 3; ouabain; peruvoside; phenylmercuric acetate; primaquine; propachlor; pyrithione zinc; cardenolide; antineoplastic activity; cancer cell culture; continuous infusion; cytotoxicity; drug potency; drug structure; enzyme activation; food and drug administration; high throughput screening; immunofluorescence; in vitro study; osmotic minipump; retinoblastoma; tumor regression; tumor xenograft; drug effect; drug resistance; drug screening; enzymology; institute for cancer research mouse; pathology; retina tumor; tumor cell culture; cardenolides; dose-response relationship, drug; drug resistance, neoplasm; drug screening assays, antitumor; mice, inbred icr; mice, scid; retinal neoplasms; tumor cells, cultured; xenograft model antitumor assays |
| Journal Title: | Investigative Ophthalmology & Visual Science (IOVS) |
| Volume: | 50 |
| Issue: | 7 |
| ISSN: | 0146-0404 |
| Publisher: | Association for Research in Vision and Ophthalmology |
| Date Published: | 2009-01-01 |
| Start Page: | 3065 |
| End Page: | 3073 |
| Language: | English |
| DOI: | 10.1167/iovs.08-3158 |
| PUBMED: | 19151399 |
| PROVIDER: | scopus |
| PMCID: | PMC3617409 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 4" - "Export Date: 30 November 2010" - "CODEN: IOVSD" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
-
389Abramson -
101Djaballah -
40Antczak -
343De Stanchina -
28Radu
Related MSK Work