Immunohistochemical study of the expression of EXON11-containing μ opioid receptor variants in mouse brain Journal Article
| Authors: | Abbadie, C.; Pan, Y. X.; Pasternak, G. W. |
| Article Title: | Immunohistochemical study of the expression of EXON11-containing μ opioid receptor variants in mouse brain |
| Abstract: | The cloned μ opioid receptor MOR-1 undergoes alternative splicing. Extensive 3′-splicing downstream from exon 3 leads to a number of C-terminal splice variants that are differentially expressed within the CNS. Recently, 5′-splicing has been observed with eight additional variants containing exon 11, a new exon located approximately 10 kb upstream from exon 1 that is under the control of a different promoter located even further upstream. Three of these variants generate the same protein as MOR-1 itself, but under the control of the new exon 11 promoter. Three variants in which exon 11 is translated have been identified within the brain, including MOR-1G, MOR-1M and MOR-1N. The present paper defines immunohistochemically the distribution of these variants using an exon 11-specific antiserum. The expression of exon 11-like immunoreactivity (-LI) was seen primarily in the olfactory tubercle, caudate-putamen, globus pallidus and substantia nigra. We did not observe exon 11-LI in a number of regions expressing MOR-1. Within the caudate-putamen, the general pattern of labeling was diffuse, in contrast to the pattern seen with an exon 4-generated antiserum that labels MOR-1 itself. However, we did observe in the caudate-putamen co-expression of exon 4- and exon 11-LI in cells that were apposed to dopaminergic terminals. These results provide new insights regarding the potential physiological significance of these exon 11-containing variants. © 2004 IBRO. Published by Elsevier Ltd. All rights reserved. |
| Keywords: | immunohistochemistry; controlled study; protein expression; exon; exons; nonhuman; protein localization; mouse; animals; mice; animal tissue; cell line; immunoreactivity; gene expression regulation; brain; presynaptic terminals; nucleotide sequence; alternative splicing; antibody specificity; morphine; antiserum; mu opiate receptor; brain region; receptors, opioid, mu; protein isoforms; dopamine; dopaminergic nerve cell; corpus striatum; keyhole limpet hemocyanin; olfactory bulb; opiate receptor; putamen; brain nerve cell; nerve ending; mor-1; promoter regions (genetics); globus pallidus; periaqueductal gray; chemical labeling; substantia nigra; splice variant; klh; olfactory pathways; mop; hek; caudate nucleus; humans; male; priority journal; article; -li; -like immunoreactivity; cdna clone encoding a μ opioid receptor; human embryonic kidney; pag; pb; phosphate buffer; th |
| Journal Title: | Neuroscience |
| Volume: | 127 |
| Issue: | 2 |
| ISSN: | 0306-4522 |
| Publisher: | Pergamon-Elsevier Science Ltd |
| Date Published: | 2004-01-01 |
| Start Page: | 419 |
| End Page: | 430 |
| Language: | English |
| DOI: | 10.1016/j.neuroscience.2004.03.033 |
| PROVIDER: | scopus |
| PUBMED: | 15262332 |
| DOI/URL: | |
| Notes: | Neuroscience -- Cited By (since 1996):21 -- Export Date: 16 June 2014 -- Source: Scopus |
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